化学
荧光
斑马鱼
扫描电镜
极性(国际关系)
细胞器
脂滴
生物物理学
共焦显微镜
细胞生物学
细胞内
共焦
显微镜
生物化学
病理
激光器
受激发射
光学
基因
生物
量子力学
数学
几何学
医学
物理
细胞
作者
Xiao Zhang,Xingyue Li,Wenting Xu,Shuxin Li,Xucong Zhou,Zeng‐Yu Wang,Quan Zhang,Jin Zhou
出处
期刊:Talanta
[Elsevier]
日期:2024-10-01
卷期号:278: 126452-126452
标识
DOI:10.1016/j.talanta.2024.126452
摘要
Septic cardiomyopathy (SCM) is the main cause of death in critically ill patients with sepsis. However, its definitive pathogenic mechanisms remain to be elucidated. Lipid droplets (LDs) are important sub-organelles that store lipids and participate in intracellular lipid metabolism. Abnormal aggregation and altered polarity of LDs are associated with the development of several cardiac diseases. To date, visualization of abnormal polarity in models of SCM has not been achieved. Herein, we designed and synthesized the probe BDP-551, a polarity-sensitive probe possessing a donor-π-acceptor (D-π-A) structure. BDP-551 exhibits excellent photostability, high LDs targeting, near-infrared (NIR) emission (up to 678 nm) and strong polarity sensitivity. With the help of confocal imaging microscopy, the BDP-551 was able to detect the polarity changes induced in the SCM model cells and visualize the yolk sac region in hypoxic as well as inflamed living zebrafish. In addition, the BDP-551 has been successfully applied to visualize the polarity changes of mice hearts with SCM, proving a decrease of microenvironmental polarity in the development of SCM. Therefore, BDP-551 in this study can be used as a reliable tool to investigate polarity fluctuations and provide new insights into the associated pathogenic and therapeutic mechanisms on SCM.
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