生物
腹索神经
神经科学
运动神经元
神经系统
脊髓
再生(生物学)
幼虫
模式生物
功能(生物学)
解剖
进化生物学
细胞生物学
生态学
基因
遗传学
作者
Tho Huu Nguyen,Rosario Vicidomini,Saumitra Dey Choudhury,Tae Hee Han,Dragan Maric,Thomas Brody,Mihaela Serpe
标识
DOI:10.1016/j.devcel.2024.03.016
摘要
The Drosophila larval ventral nerve cord (VNC) shares many similarities with the spinal cord of vertebrates and has emerged as a major model for understanding the development and function of motor systems. Here, we use high-quality scRNA-seq, validated by anatomical identification, to create a comprehensive census of larval VNC cell types. We show that the neural lineages that comprise the adult VNC are already defined, but quiescent, at the larval stage. Using fluorescence-activated cell sorting (FACS)-enriched populations, we separate all motor neuron bundles and link individual neuron clusters to morphologically characterized known subtypes. We discovered a glutamate receptor subunit required for basal neurotransmission and homeostasis at the larval neuromuscular junction. We describe larval glia and endorse the general view that glia perform consistent activities throughout development. This census represents an extensive resource and a powerful platform for future discoveries of cellular and molecular mechanisms in repair, regeneration, plasticity, homeostasis, and behavioral coordination.
科研通智能强力驱动
Strongly Powered by AbleSci AI