间充质干细胞
活体显微镜检查
肺
体内
免疫系统
炎症
生物
内皮干细胞
病理
癌症研究
体外
免疫学
细胞生物学
医学
内科学
生物技术
生物化学
作者
Chen Wang,Kamal Hezam,Enze Fu,Kai Pan,Yue Liu,Zongjin Li
标识
DOI:10.1016/j.yexcr.2024.114013
摘要
Mesenchymal stem cells (MSCs) have been widely used to treat various inflammatory and immune-related diseases in preclinical and clinical settings. Intravital microscopy (IVM) is considered the gold standard for investigating pathophysiological conditions in living animals. However, the potential for real-time monitoring of MSCs in the pulmonary microenvironment remains underexplored. In this study, we first constructed a lung window and captured changes in the lung at the cellular level under both inflammatory and noninflammatory conditions with a microscope. We further investigated the dynamics and effects of MSCs under two different conditions. Meanwhile, we assessed the alterations in the adhesive capacity of vascular endothelial cells in vitro to investigate the underlying mechanisms of MSC retention in an inflammatory environment. This study emphasizes the importance of the "lung window" for live imaging of the cellular behavior of MSCs by vein injection. Moreover, our results revealed that the upregulation of vascular cell adhesion molecule 1 (VCAM1) in endothelial cells post-inflammatory injury could enhance MSC retention in the lung, further ameliorating acute lung injury. In summary, intravital microscopy imaging provides a practical method to investigate the therapeutic effects of MSCs in acute lung injury.
科研通智能强力驱动
Strongly Powered by AbleSci AI