OsCaM1‐1 Is Responsible for Salt Tolerance by Regulating Na+/K+ Homoeostasis in Rice

平衡 突变体 基因 钙调蛋白 转录因子 化学 盐(化学) 基因表达 野生型 生物 细胞生物学 生物化学 物理化学
作者
Siqi Wei,Mingjiong Chen,Fengyue Wang,Yishan Tu,Yunfeng Xu,Liangbo Fu,Fanrong Zeng,Guoping Zhang,Dezhi Wu,Qiufang Shen
出处
期刊:Plant Cell and Environment [Wiley]
标识
DOI:10.1111/pce.15212
摘要

ABSTRACT Calmodulin, a highly conserved calcium‐binding protein, plays a crucial role in response to salt stress. Previous studies investigated sequence and function of calmodulin members in some plants, but their roles in rice have not been fully elucidated. Three OsCaM1 genes namely OsCaM1‐1/2/3 encode the same OsCaM1 protein. Here, we found that OsCaM1‐1 had significantly higher expression than the other two genes under salt stress. After 4 weeks of exposure to 75 mM NaCl, OsCaM1‐1 overexpressed mutants showed higher salt tolerance, while knocked‐out mutants exhibited lower salt tolerance, compared to the wild type. Moreover, the oscam1‐1 mutants had higher Na + concentration and Na + /K + ratio in both shoots and roots, less instantaneous K + and Ca 2+ fluxes in roots, compared to wild type under salt stress, indicating the involvement of OsCaM1‐1 in regulation of Na + and K + homoeostasis via Ca 2+ signal. RNA‐seq analysis identified 452 differentially expressed genes (DEGs) regulated by OsCaM1‐1 and salt stress, and they were mainly enriched in nucleus DNA‐binding activities, including ABI5 , WRKY76 , WRKY48 and bHLH120 transcription factors. Knockout of OsCaM1‐1 also modulated the expression of Na + transporters, including HKT1;1 , HKT1;5 , SOS1 , NHX1 and NHX4 . In conclusion, OsCaM1‐1 positively regulates salt tolerance in rice through mediating ion homoeostasis.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
情怀应助成就芒果tv采纳,获得20
1秒前
2秒前
yangr完成签到,获得积分20
5秒前
小谢发布了新的文献求助10
5秒前
Jasper应助ggM采纳,获得10
6秒前
慕青应助ggM采纳,获得10
6秒前
袁琴发布了新的文献求助10
7秒前
KWang应助菠萝萝萝王子采纳,获得10
7秒前
丘比特应助YYY666采纳,获得10
9秒前
简单山水发布了新的文献求助10
9秒前
9秒前
大模型应助阳阳采纳,获得10
13秒前
zz想吃鱼发布了新的文献求助10
13秒前
13秒前
简单山水完成签到,获得积分10
17秒前
18秒前
19秒前
drift发布了新的文献求助10
21秒前
22秒前
许中天发布了新的文献求助10
22秒前
善学以致用应助奋斗小真采纳,获得10
24秒前
李玉琼完成签到,获得积分10
25秒前
阳阳发布了新的文献求助10
25秒前
冷酷语蝶发布了新的文献求助10
27秒前
SciGPT应助dilibolaba采纳,获得10
27秒前
Yangzx发布了新的文献求助10
28秒前
zho发布了新的文献求助10
29秒前
31秒前
32秒前
32秒前
汉堡包应助kk采纳,获得10
32秒前
33秒前
小谢完成签到,获得积分10
35秒前
36秒前
38秒前
38秒前
张凯完成签到,获得积分10
38秒前
38秒前
39秒前
saidosiuceyiwo完成签到 ,获得积分10
40秒前
高分求助中
좌파는 어떻게 좌파가 됐나:한국 급진노동운동의 형성과 궤적 2500
Sustainability in Tides Chemistry 1500
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
CLSI EP47 Evaluation of Reagent Carryover Effects on Test Results, 1st Edition 800
Threaded Harmony: A Sustainable Approach to Fashion 799
Livre et militantisme : La Cité éditeur 1958-1967 500
Retention of title in secured transactions law from a creditor's perspective: A comparative analysis of selected (non-)functional approaches 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3053902
求助须知:如何正确求助?哪些是违规求助? 2711045
关于积分的说明 7424610
捐赠科研通 2355580
什么是DOI,文献DOI怎么找? 1247273
科研通“疑难数据库(出版商)”最低求助积分说明 606339
版权声明 596012