细胞凋亡
细胞毒性
活力测定
MTT法
细胞毒性T细胞
癌细胞
程序性细胞死亡
细胞
体内
化学
体外
活性氧
细胞生物学
生物
传统医学
药理学
癌症
生物化学
医学
生物技术
遗传学
出处
期刊:Toxicology Research
[Oxford University Press]
日期:2024-07-01
卷期号:13 (4)
标识
DOI:10.1093/toxres/tfae124
摘要
Abstract Natural products based on their significant anti-cancer potencies have been used in cancer treatment. A natural blend of triterpenoid saponins derived from the horse chestnut (Aesculus hippocastanum L.), has been investigated in various diseases based on its main active ingredient escin. Herein, we examined the potential antiproliferative, proapoptotic, and cytotoxic activities of escin on human skin melanoma (CHL-1) cells. Cytotoxicity of escin was determined by MTT assay. Morphological changes were detected by confocal microscopy and ultrastructural changes by transmission electron microscopy studies. Phosphatidylserine translocation assay, Bcl-2 activation assessment, and oxidative stress analysis were used to determine the cell death mode of the cells. The results showed that escin reduced cell viability in a dose-dependent manner within 24 h of exposure and was highly cytotoxic at lower concentrations (IC50 value 6 μg/mL). Escin inactivated Bcl-2 signaling and triggered apoptosis by increasing the reactive oxygen species and by causing morphological and ultrastructural changes that implicate to the proapoptotic activity. Escin has been found to exert high potential for an anti-cancer drug following further in vitro and in vivo investigations.
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