生物
聚腺苷酸
相扑蛋白
拟南芥
细胞生物学
计算生物学
裂解和多聚腺苷酸化特异性因子
遗传学
基因表达
突变体
基因
泛素
作者
Zhibo Yu,Wei Wang,Cheng Zhang,Qiuna Zhan,Leqian Shi,Bing Song,Danlu Han,Jieming Jiang,Junwen Huang,Xiaolin Ou,Zhonghui Zhang,Jianbin Lai,Qingshun Quinn Li,Chengwei Yang
标识
DOI:10.1016/j.molp.2024.07.011
摘要
Under warm temperatures, plants adjust their morphologies for environmental adaption via precise gene expression regulation. However, the function and regulation of alternative polyadenylation (APA), an important fine-tuning of gene expression, remains unknown in plant thermomorphogenesis. In this study, we found that SUMOylation, a critical post-translational modification, is induced by a long-term treatment at warm temperatures via a SUMO ligase SIZ1 in Arabidopsis. Disruption of SIZ1 altered the global usage of polyadenylation signals and affected the APA dynamic of thermomorphogenesis-related genes. CPSF100, a key subunit of the CPSF complex for polyadenylation regulation, is SUMOylated by SIZ1. Importantly, we demonstrated that SUMOylation is essential for the function of CPSF100 in genome-wide polyadenylation site choice during thermomorphogenesis. Further analyses revealed that the SUMO conjugation on CPSF100 attenuates its interaction with two isoforms of its partner CPSF30, increasing the nuclear accumulation of CPSF100 for polyadenylation regulation. In summary, our study uncovers a regulatory mechanism of APA via SIZ1-mediated SUMOylation in plant thermomorphogenesis.
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