循环肿瘤细胞
星团(航天器)
前列腺癌
癌症研究
乳腺癌
黑色素瘤
癌症
生物
转移
计算生物学
遗传学
计算机科学
程序设计语言
作者
A. Fatih Sarioglu,Nicola Aceto,Nikola Kojić,Maria C. Donaldson,Mahnaz Zeinali,Bashar Hamza,Amanda Engstrom,Huili Zhu,Tilak Sundaresan,David T. Miyamoto,Xi Luo,Aditya Bardia,Ben S. Wittner,Sridhar Ramaswamy,Toshi Shioda,David T. Ting,Shannon L. Stott,Ravi Kapur,Shyamala Maheswaran,Daniel A. Haber,Mehmet Toner
出处
期刊:Nature Methods
[Springer Nature]
日期:2015-05-18
卷期号:12 (7): 685-691
被引量:631
摘要
Cancer cells metastasize through the bloodstream either as single migratory circulating tumor cells (CTCs) or as multicellular groupings (CTC clusters). Existing technologies for CTC enrichment are designed to isolate single CTCs, and although CTC clusters are detectable in some cases, their true prevalence and significance remain to be determined. Here we developed a microchip technology (the Cluster-Chip) to capture CTC clusters independently of tumor-specific markers from unprocessed blood. CTC clusters are isolated through specialized bifurcating traps under low-shear stress conditions that preserve their integrity, and even two-cell clusters are captured efficiently. Using the Cluster-Chip, we identified CTC clusters in 30-40% of patients with metastatic breast or prostate cancer or with melanoma. RNA sequencing of CTC clusters confirmed their tumor origin and identified tissue-derived macrophages within the clusters. Efficient capture of CTC clusters will enable the detailed characterization of their biological properties and role in metastasis.
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