转染
体内
细胞毒性
基因传递
体外
荧光素酶
分子生物学
质粒
遗传增强
生物
化学
材料科学
DNA
生物化学
基因
生物技术
作者
Hong Yao,Samuel S. Ng,Wesley Owen Tucker,Yuk-Kai-Tiu Tsang,Kwan Man,Xiaomei Wang,Bkc Chow,Hsing-Jien Kung,Guping Tang,Marie C.M. Lin
出处
期刊:Biomaterials
[Elsevier]
日期:2009-10-01
卷期号:30 (29): 5793-5803
被引量:102
标识
DOI:10.1016/j.biomaterials.2009.06.051
摘要
The success of gene therapy relies on a safe and effective gene delivery system. In this communication, we describe the use of folate grafted PEI(600)-CyD (H(1)) as an effective polyplex-forming plasmid delivery agent with low toxicity. The structures of the polymer and polyplex were characterized, and the in vitro transfection efficiency, cytotoxicity, and in vivo transfection of H(1) were examined. We found that folate molecules were successfully grafted to PEI(600)-CyD. At N/P ratios between 5 and 30, the resulting H(1)/DNA polyplexes had diameters less than 120 nm and zeta potentials less than 10 mV. In various tumor cell lines examined (U138, U87, B16, and Lovo), the in vitro transfection efficiency of H(1) was more than 50%, which could be improved by the presence of fetal bovine serum or albumin. The cytotoxicity of H(1) was significantly less than high molecular weight PEI-25 kDa. Importantly, in vivo optical imaging showed that the efficiency of H(1)-mediated transfection (50 microg luciferase plasmid (pLuc), N/P ratio=20/1) was comparable to that of adenovirus-mediated luciferase transduction (1 x 10(9) pfu) in melanoma-bearing mice, and it did not induce any toxicity in the tumor tissue. These results clearly show that H(1) is a safe and effective polyplex-forming agent for both in vitro and in vivo transfection of plasmid DNA and its application warrants further investigation.
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