壳聚糖
共价键
自愈水凝胶
葡萄糖氧化酶
化学
电解
电化学
化学工程
流体学
阳极
生物传感器
沉积(地质)
高分子化学
电极
纳米技术
材料科学
有机化学
电解质
生物化学
古生物学
物理化学
航空航天工程
工程类
生物
沉积物
作者
Kelsey M. Gray,Benjamin D. Liba,Yifeng Wang,Yi Cheng,Gary W. Rubloff,William E. Bentley,Alexandra Montembault,Isabelle Royaud,Laurent David,Eunkyoung Kim
出处
期刊:Biomacromolecules
[American Chemical Society]
日期:2012-03-26
卷期号:13 (4): 1181-1189
被引量:82
摘要
The electrodeposition of hydrogels provides a programmable means to assemble soft matter for various technological applications. We report an anodic method to deposit hydrogel films of the aminopolysaccharide chitosan. Evidence suggests the deposition mechanism involves the electrolysis of chloride to generate reactive chlorine species (e.g., HOCl) that partially oxidize chitosan to generate aldehydes that can couple covalently with amines (presumably through Schiff base linkages). Chitosan's anodic deposition is controllable spatially and temporally. Consistent with a covalent cross-linking mechanism, the deposited chitosan undergoes repeated swelling/deswelling in response to pH changes. Consistent with a covalent conjugation mechanism, proteins could be codeposited and retained within the chitosan film even after detergent washing. As a proof-of-concept, we electroaddressed glucose oxidase to a side-wall electrode of a microfabricated fluidic channel and demonstrated this enzyme could perform electrochemical biosensing functions. Thus, anodic chitosan deposition provides a reagentless, single-step method to electroaddress a stimuli-responsive and biofunctionalized hydrogel film.
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