[Overview of Multiplex Immunohistochemistry and Immunofluorescence Techniques 
in the Lung Cancer Immunotherapy].

Lung cancer is the most common malignancy and is a major public health problem worldwide. Programmed cell death receptor 1 (PD-1)/programmed cell death ligand 1 (PD-L1) inhibitors provide a new treatment strategy for non-small cell lung cancer (NSCLC). The existing biomarkers all have advantages and defects in selecting patients who benefit from immunotherapy. The multiplex immunohistochemistry/immunofluorescence (mIHC/IF) provides multiplex staining, allows comprehensive studies of cellular composition, cellular functions and cell-cell interactions. A number of studies have used mIHC/IF to explore specific immune cells in tumor immune microenvironment (TIME) and found that it is helpful for clinical prognosis and efficacy prediction in patients with lung cancer. In the era of immunotherapy for lung cancer, this technique has a bright future in translational research and clinical practice. The research progress of mIHC/IF detection in lung cancer immunotherapy is summarized in this review. .【中文题目：多色标记免疫组织化学染色和免疫荧光染色 在肺癌免疫治疗中的研究进展】 【中文摘要：肺癌是目前临床上最常见的恶性肿瘤，严重威胁着患者的生命健康及生活质量。程序性细胞死亡受体1（programmed cell death receptor 1, PD-1）及其配体（programmed cell death ligand 1, PD-L1）抑制剂为非小细胞肺癌（non-small cell lung cancer, NSCLC）患者提供了新的治疗策略。现有的生物标志物检测对准确选择免疫治疗受益的患者均有一定的价值，但都存在着局限性。多标记免疫组织化学/免疫荧光（multiplex immunohistochemistry/immunofluorescence, mIHC/IF）技术允许在单一组织切片上同时检测多个抗体，并对细胞组成、细胞功能和细胞-细胞相互作用进行全面研究。国内外已有大量研究使用mIHC/IF技术对肿瘤免疫微环境（tumor immune microenvironment, TIME）下特异性免疫细胞群进行了探索，发现其有助于肺癌患者临床预后判断及疗效预测。肺癌免疫治疗时代，这项技术在转化研究和临床实践中均具有良好的应用前景。本文就mIHC/IF检测方法在肺癌免疫治疗中的研究进展进行了总结和展望。 】 【中文关键词：肺肿瘤；多色标记；免疫组织化学；免疫荧光；免疫治疗】.