This chapter deals with the structural chemistry and biosynthetic pathways of the major classes of steroid hormones. All have a complicated structure of fused rings which can be modified by functional group substitution at many points. Furthermore, the presence of asymmetric carbon atoms introduces steric modifications and isomeric possibilities. The reader will find it prudent to first grasp the essential features of the steroid structures and relationships before attempting to delve into a consideration of their specific hormonal activities in later chapters. Then, when so doing, it may be helpful to turn back to the appropriate portion of this chapter to further heighten understanding of the structures of the hormones under review.