磷酸化
蛋白质磷酸化
计算机科学
化学
计算生物学
蛋白激酶A
生物
生物化学
作者
Dequan Zhang,Xin Li,Li Chen,Chengli Hou,Zhenyu Wang
出处
期刊:Springer eBooks
[Springer Nature]
日期:2020-01-01
卷期号:: 11-21
标识
DOI:10.1007/978-981-15-9441-0_2
摘要
With the advancement of technical approaches, signaling pathways have been continuously discovered in numerous biological processes. Majority of these signaling pathways include the protein phosphorylation, which is a main post-translational modification affecting the structure and function of proteins within the cell. Consequently, determining the locations and extent of the protein phosphorylation of any specific protein or set of proteins, either qualitatively or quantitatively, has become a routine and extremely vital step in many fields of life sciences, and recently in meat science as well. However, when choosing the method to detect protein phosphorylation, it can be different depending on several aspects, particularly under consideration of the availability of the related reagents and equipment. Although different methods have their own advantages, but this is also a matter of fact that every method is associated with several drawbacks such as poor specificity, low sensitivity, safety issues, or intensive labor. In this chapter, we outlined the routine methods used to detect the protein phosphorylation and discussed their advantages and disadvantages, especially focusing on the usage of gel and liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) techniques to identify phosphorylated proteins in meat science field.
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