白粉病
索引
生物
甜瓜
遗传标记
分子标记
标记辅助选择
遗传学
黄瓜
聚合酶链反应
基因座(遗传学)
裂解扩增多态序列
数量性状位点
基因型
园艺
基因
限制性片段长度多态性
单核苷酸多态性
作者
Yu-Ri Choi,Jae Yong Lee,Seongbin Hwang,Hyun Uk Kim
出处
期刊:Agronomy
[MDPI AG]
日期:2020-08-28
卷期号:10 (9): 1274-1274
被引量:5
标识
DOI:10.3390/agronomy10091274
摘要
Powdery mildew (PM) is a fungal disease occurring in both field and greenhouse conditions worldwide. It infects many plant species and reduces both the productivity and quality of crops. Melon (Cucumis melo L.) is an economically important crop. In order to develop a molecular marker that can be used more conveniently in the development of PM-resistant melon using MR-1 melon resources, the previously reported cleaved amplified polymorphic sequence (CAPS) marker was improved with a length polymorphism PCR marker. Two cleaved CAPS markers—BSA12-LI3ECORI and BSA12-LI4HINFI—associated with BPm12.1, a major quantitative trait locus (QTL) corresponding to the PM resistance of MR-1, have been reported. In this study, we found that in the BSA12-LI3ECORI CAPS marker specifically, a 41 bp deletion was present in the PCR DNA region of the MR-1 melon genome. A new marker capable of distinguishing polymerase chain reaction (PCR) length polymorphism was produced using insertion-deletion (InDel) information in this region. This PCR-based InDel marker distinguished the genotypes of PM-resistant MR-1, PM-susceptible Top Mark, and their F1 progeny. These results suggest that this InDel marker could be used to develop PM-resistant melon varieties based on MR-1.
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