Method to Isolate Mesenchymal‐Like Cells from Wharton's Jelly of Umbilical Cord

沃顿果冻 间充质干细胞 脐带 帘布衬里 干细胞 免疫学 细胞生物学 成体干细胞 医学 生物 体外 内皮干细胞 生物化学
作者
Kiran Seshareddy,Deryl L. Troyer,Mark L. Weiss
出处
期刊:Methods in Cell Biology [Elsevier]
卷期号:: 101-119 被引量:148
标识
DOI:10.1016/s0091-679x(08)00006-x
摘要

The umbilical cord is a noncontroversial source of mesenchymal-like stem cells. Mesenchymal-like cells are found in several tissue compartments of the umbilical cord, placenta, and decidua. Here, we confine ourselves to discussing mesenchymal-like cells derived from Wharton's Jelly, called umbilical cord matrix stem cells (UCMSCs). Work from several laboratories shows that these cells have therapeutic potential, possibly as a substitute cell for bone marrow-derived mesenchymal stem cells for cellular therapy. There have been no head-to-head comparisons between mesenchymal cells derived from different sources for therapy; therefore, their relative utility is not understood. In this chapter, the isolation protocols of the Wharton's Jelly-derived mesenchymal cells are provided as are protocols for their in vitro culturing and storage. The cell culture methods provided will enable basic scientific research on the UCMSCs. Our vision is that both umbilical cord blood and UCMSCs will be commercially collected and stored in the future for preclinical work, public and private banking services, etc. While umbilical cord blood banking standard operating procedures exist, the scenario mentioned above requires clinical-grade UCMSCs. The hurdles that have been identified for the generation of clinical-grade umbilical cord-derived mesenchymal cells are discussed.
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