木聚糖酶
英特因
纤维素乙醇
玉米秸秆
生物量(生态学)
木糖
原材料
化学
酶水解
水解
食品科学
生物技术
生物燃料
木质纤维素生物量
农学
纤维素
生物化学
酶
生物
发酵
基因
有机化学
核糖核酸
RNA剪接
作者
Binzhang Shen,Xiao‐Man Sun,Xiao Zuo,Taran Shilling,James R. Apgar,Mary Ross,Oleg Bougri,Vladimir Samoylov,Matthew H. Parker,Elaina Hancock,Héctor A. Lucero,Benjamin N. Gray,Nathan A. Ekborg,Dongcheng Zhang,Jeremy C. S. Johnson,G. Lázár,R. Michael Raab
摘要
Plant cellulosic biomass is an abundant, low-cost feedstock for producing biofuels and chemicals. Expressing cell wall-degrading (CWD) enzymes (e.g. xylanases) in plant feedstocks could reduce the amount of enzymes required for feedstock pretreatment and hydrolysis during bioprocessing to release soluble sugars. However, in planta expression of xylanases can reduce biomass yield and plant fertility. To overcome this problem, we engineered a thermostable xylanase (XynB) with a thermostable self-splicing bacterial intein to control the xylanase activity. Intein-modified XynB (iXynB) variants were selected that have 60% enzymatic activity upon intein self-splicing at temperatures >59 °C. Greenhouse-grown xynB maize expressing XynB has shriveled seeds and low fertility, but ixynB maize had normal seeds and fertility. Processing dried ixynB maize stover by temperature-regulated xylanase activation and hydrolysis in a cocktail of commercial CWD enzymes produced >90% theoretical glucose and >63% theoretical xylose yields.
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