重编程
生物
核糖核酸
RNA剪接
Wnt信号通路
计算生物学
RNA结合蛋白
细胞生物学
基因表达
信号转导
基因表达调控
基因
遗传学
作者
Stephanie J. Culler,Kevin G. Hoff,Christina D. Smolke
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2010-11-26
卷期号:330 (6008): 1251-1255
被引量:249
标识
DOI:10.1126/science.1192128
摘要
Synthetic genetic devices that interface with native cellular pathways can be used to change natural networks to implement new forms of control and behavior. The engineering of gene networks has been limited by an inability to interface with native components. We describe a class of RNA control devices that overcome these limitations by coupling increased abundance of particular proteins to targeted gene expression events through the regulation of alternative RNA splicing. We engineered RNA devices that detect signaling through the nuclear factor κB and Wnt signaling pathways in human cells and rewire these pathways to produce new behaviors, thereby linking disease markers to noninvasive sensing and reprogrammed cellular fates. Our work provides a genetic platform that can build programmable sensing-actuation devices enabling autonomous control over cellular behavior.
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