Developmentally Imprinted Genes as Markers for Bladder Tumor Progression

No AccessJournal of UrologyInvestigative Urology1 Jun 1996Developmentally Imprinted Genes as Markers for Bladder Tumor Progression Mark J. Cooper, Martin Fischer, Dymitr Komitowski, Alexander Shevelev, Ekkehard Schulze, Ilana Ariel, Mark L. Tykocinski, Stela Miron, Joseph Ilan, Nathan De Groot, and Abraham Hochberg Mark J. CooperMark J. Cooper , Martin FischerMartin Fischer , Dymitr KomitowskiDymitr Komitowski , Alexander ShevelevAlexander Shevelev , Ekkehard SchulzeEkkehard Schulze , Ilana ArielIlana Ariel , Mark L. TykocinskiMark L. Tykocinski , Stela MironStela Miron , Joseph IlanJoseph Ilan , Nathan De GrootNathan De Groot , and Abraham HochbergAbraham Hochberg View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)66120-2AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Developmentally imprinted genes, such as H19 and insulin-like growth factor-II (IGF-II), play an important role during human embryogenesis and also have been implicated in the pathogenesis of embryonal tumors of childhood. Since H19 is expressed in human fetal bladder, we evaluated 35 bladder carcinomas for H19 expression by in situ hybridization analysis and correlated expression with tumor grade. As a prelude to gene transfer studies to determine if H19 is a bladder tumor oncogene, we also evaluated bladder cell lines for expression of H19, IGF-II, IGF-I and the type I IGF receptor. Materials and Methods: H19 expression was evaluated by in situ hybridization analysis in bladder tumor specimens. Northern analysis was used to evaluate the expression of H19, IGF-II, IGF-I and the type I IGF receptor in bladder cell lines. Results: H19 was expressed preferentially in advanced stage tumors: 2 of 12 grade I tumors were H19 positive, whereas 9 of 11 grade II and 7 of 10 grade III tumors expressed H19 (p = 0.004). Additionally, 6 of 6 carcinoma in situ tumors were H19 positive, whereas normal bladder mucosa cells were H19 negative. We found that 3 of 11 cell lines (HT-1376, HT-1197 and 5637) express high levels of H19 mRNA, and each of these cell lines and J82 also express IGF-II. All cell lines examined expressed the type I IGF receptor, whereas there was no detectable IGF-I mRNA. Conclusions: These data demonstrate that H19 is an oncodevelopmental marker of bladder tumor progression and raise the possibility that H19 may have oncogenic properties in bladder cancer. 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Google Scholar From the Departments of Medicine and Pathology, Case Western Reserve University School of Medicine, Cleveland, Ohio; the German Cancer Research Center, Heidelberg, Germany; the Department of Pathology, Hadassah University Hospital, Mount Scopus, and the Department of Biological Chemistry, The Silberman Institute of Life Sciences, Hebrew University, Jerusalem, Israel.© 1996 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 155Issue 6June 1996Page: 2120-2127 Advertisement Copyright & Permissions© 1996 by American Urological Association, Inc.MetricsAuthor Information Mark J. Cooper More articles by this author Martin Fischer More articles by this author Dymitr Komitowski More articles by this author Alexander Shevelev More articles by this author Ekkehard Schulze More articles by this author Ilana Ariel More articles by this author Mark L. Tykocinski More articles by this author Stela Miron More articles by this author Joseph Ilan More articles by this author Nathan De Groot More articles by this author Abraham Hochberg More articles by this author Expand All Advertisement PDF downloadLoading ...