染色体构象捕获
染色体
染色质
生物
人口
减数分裂
酿酒酵母
遗传学
基因组
酵母
计算生物学
DNA
基因
社会学
增强子
人口学
基因表达
作者
Job Dekker,Karsten Rippe,Martijn Dekker,Nancy Kleckner
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2002-02-15
卷期号:295 (5558): 1306-1311
被引量:3550
标识
DOI:10.1126/science.1067799
摘要
We describe an approach to detect the frequency of interaction between any two genomic loci. Generation of a matrix of interaction frequencies between sites on the same or different chromosomes reveals their relative spatial disposition and provides information about the physical properties of the chromatin fiber. This methodology can be applied to the spatial organization of entire genomes in organisms from bacteria to human. Using the yeast Saccharomyces cerevisiae , we could confirm known qualitative features of chromosome organization within the nucleus and dynamic changes in that organization during meiosis. We also analyzed yeast chromosome III at the G 1 stage of the cell cycle. We found that chromatin is highly flexible throughout. Furthermore, functionally distinct AT- and GC-rich domains were found to exhibit different conformations, and a population-average 3D model of chromosome III could be determined. Chromosome III emerges as a contorted ring.
科研通智能强力驱动
Strongly Powered by AbleSci AI