没食子酸
化学
芦丁
槲皮素
山奈酚
抗菌剂
食品科学
DPPH
杨梅素
抗氧化剂
生物化学
有机化学
作者
M.S. Yogendra Kumar,R J Tirpude,Doddamane Thippeswamy Maheshwari,Anju Bansal,Ksipra Misra
出处
期刊:Food Chemistry
[Elsevier]
日期:2013-12-01
卷期号:141 (4): 3443-3450
被引量:82
标识
DOI:10.1016/j.foodchem.2013.06.057
摘要
Phenolic rich fraction (PRF) from Seabuckthorn leaves was prepared by sequential fractionation. Total phenolic content of PRF estimated as gallic acid equivalent was found to be 319.33±7.02 mg/g of PRF. Its major constituents gallic acid, rutin, quercetin-3-galactoside, quercetin-3-glucoside, myricetin, quercetin, kaempferol and isorhamnetin, were found in the range of 1.551-196.89 mg/g of PRF as determined by RP-HPLC. Antioxidant activity of PRF evaluated using 2,2-diphenyl-2-picrylhydrazyl, superoxide and nitric oxide scavenging assays. Reducing power of PRF increased with increasing amount of PRF; the equation of reducing power (y) and amount of PRF (x) was y=8.004x (r(2)=0.99), indicating that reducing ability correlated well with amount of PRF. Antibacterial activity of PRF, tested against certain medically important bacterial species showed growth inhibiting effect against Escherichia coli, Salmonella typhi, Shigella dysenteriae, Streptococcus pneumoniae and Staphylococcus aureus. In conclusion, PRF has potent antioxidant and broad spectrum antibacterial properties.
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