亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Anti-cancer effects of oncolytic viral therapy combined with photodynamic therapy in human pancreatic cancer cell lines.

溶瘤腺病毒 癌细胞 癌症治疗 细胞凋亡 肿瘤科 溶癌病毒 联合疗法 遗传增强
作者
Yazan S. Khaled,K. E. Wright,Alan Melcher,David Jayne
出处
期刊:The Lancet [Elsevier]
卷期号:385 被引量:6
标识
DOI:10.1016/s0140-6736(15)60371-3
摘要

Abstract Background Oncolytic viral therapy and photodynamic therapy are potential therapies for inoperable or advanced pancreatic cancer. Our aim was to investigate the anti-cancer killing effects of reovirus therapy combined with protoporphyrin IX (PpIX)-mediated photodynamic therapy on a variety of human pancreatic cancer cell lines. Methods Pancreatic cancer cell lines (PsPC-1 and BXPC-3) and a non-cancer control cell line (HEK293) were infected with reovirus serotype 3 strain Dearing (T3D) at 0, 0·1, 1, and 10 plaque-forming units (PFU) per cell for 48 h. Cells were incubated with PpIX pro-drug 5-aminolevulinic acid (5-ALA) at 0, 1, 2, 3, and 4 mM for 4 h. Then, cells were photo-irradiated for 15 min with visible red light-emitting diodes with a light-fluence of 0·54 J/cm 2 of 653 nm (PpIX optimal excitation wavelength). The killing effects of reovirus combined with PpIX-mediated photodynamic therapy were analysed in methylthiazoltetrazolium (MTT) and trypan blue assays. The effect of adding reovirus after photodynamic therapy was also assessed. The statistical significance of the difference between groups was assessed with the two-tailed Student's t test. p Findings Reovirus monotherapy induced cell death in the two pancreatic lines (mean 57% [SE 10·2] at 10 PFU per cell). PpIX-mediated PDT monotherapy induced cell death in a dose-dependent manner (mean 10% [SE 2·2], 30 [6·4], 50 [8·2], and 70 [13·2] after 1, 2, 3, and 4 mM 5-ALA, respectively). Reovirus with PpIX-mediated photodynamic therapy resulted in a significantly increased cytotoxic effect compared with reovirus monotherapy and photodynamic therapy (p=0·042) with 100% cell death observed across pancreatic cell lines with 10 PFU per cell combined with 1 and 2 mM 5-ALA. There was no difference in cytotoxicity observed between added reovirus before or after photodynamic therapy. Interpretation To our knowledge, this is the first in-vitro study to combine reovirus oncolytic viral therapy with PpIX-mediated photodynamic therapy to treat pancreatic cancer. These results show a significant additive effect in cell killing and they provide initial evidence for a novel combined therapeutic intervention. Funding National Institute for Health Research.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
脑洞疼应助吴彦祖采纳,获得10
20秒前
Dani完成签到,获得积分10
22秒前
27秒前
44秒前
46秒前
科研通AI2S应助科研通管家采纳,获得10
54秒前
烟花应助LukeLion采纳,获得10
1分钟前
机灵的衬衫完成签到 ,获得积分10
1分钟前
1分钟前
rrr发布了新的文献求助10
1分钟前
慕青应助Ni采纳,获得10
1分钟前
LukeLion发布了新的文献求助10
1分钟前
1分钟前
1分钟前
Ni发布了新的文献求助10
1分钟前
悦耳秋珊完成签到 ,获得积分10
1分钟前
1分钟前
1分钟前
1分钟前
累啊完成签到,获得积分10
1分钟前
累啊发布了新的文献求助10
1分钟前
1分钟前
过时的笙完成签到,获得积分10
1分钟前
美好乐松完成签到,获得积分0
2分钟前
wlei完成签到,获得积分10
2分钟前
YSY完成签到,获得积分10
2分钟前
大鸭子完成签到 ,获得积分10
2分钟前
2分钟前
2分钟前
2分钟前
2分钟前
2分钟前
完美世界应助科研通管家采纳,获得10
2分钟前
科研通AI2S应助科研通管家采纳,获得10
2分钟前
科研通AI2S应助科研通管家采纳,获得10
2分钟前
彭于晏完成签到,获得积分10
2分钟前
3分钟前
menghua完成签到,获得积分10
3分钟前
juejue333完成签到,获得积分10
3分钟前
3分钟前
高分求助中
The late Devonian Standard Conodont Zonation 2000
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 2000
The Lali Section: An Excellent Reference Section for Upper - Devonian in South China 1500
Very-high-order BVD Schemes Using β-variable THINC Method 890
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 800
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 800
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3256852
求助须知:如何正确求助?哪些是违规求助? 2898987
关于积分的说明 8303179
捐赠科研通 2568204
什么是DOI,文献DOI怎么找? 1394935
科研通“疑难数据库(出版商)”最低求助积分说明 652924
邀请新用户注册赠送积分活动 630649