骨关节炎
软骨细胞
小RNA
免疫细胞化学
污渍
软骨
医学
退行性疾病
癌症研究
细胞生物学
生物信息学
免疫学
生物
内科学
疾病
病理
基因
解剖
遗传学
替代医学
标识
DOI:10.1016/j.biopha.2019.109186
摘要
Osteoarthritis is a degenerative joint disease, worldwide, and its underlying molecular mechanisms are still poorly understood. MicroRNAs are important regulators of diverse biological processes, including osteoarthritis. In this study, we showed that miR-103 was deregulated in osteoarthritis patients. We performed CCK8 and colony formation assay and found that miR-103 inhibited chondrocyte proliferation. We also found that miR-103 inhibited chondrocyte formation and maturation by RT-PCR, western blotting, and immunocytochemistry. Inhibition of miR-103 suppressed production of the catabolic factors and pro-inflammatory cytokines induced by IL-1β in chondrocytes. Finally, we found that Sox6 was a direct target of miR-103 and participated in osteoarthritis development. In summary, we demonstrated that miR-103 contributed to osteoarthritis development by directly targeting and inhibiting the expression of Sox6. Regulation of miR-103 expression in human chondrocytes may be an effective treatment for osteoarthritis.
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