原代培养
小学(天文学)
低温保存
体外
生物
神经元
神经科学
细胞生物学
胚胎
生物化学
天文
物理
作者
Francesca Pischedda,Giovanni Piccoli
出处
期刊:Bio-protocol
[Bio-Protocol]
日期:2019-01-01
卷期号:9 (12)
被引量:2
标识
DOI:10.21769/bioprotoc.3270
摘要
Primary neuronal culture from rodents is a key tool in neurobiology.However, the preparation of primary cultures requires precise planning, starting from animal mating.Furthermore, each preparation generates a high amount of cells that eventually go wasted.The possibility to cryopreserve primary neural cells represents a resource for in vitro studies and significantly reduces the sacrifice of animals.Here we describe that Neurostore buffer supports the cryopreservation of primary neurons.
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