流式细胞术
免疫系统
细胞仪
生物
病理
免疫学
细胞生物学
医学
作者
Felipe Gálvez‐Cancino,Ernesto López,Álvaro Lladser
出处
期刊:Methods in molecular biology
日期:2019-01-01
卷期号:: 217-222
被引量:3
标识
DOI:10.1007/978-1-4939-8979-9_16
摘要
The heterogeneity and complexity of nonlymphoid tissues has become a major obstacle for the study of immune populations. For this reason, the generation of highly reproducible protocols that allow the analysis of immune cells in these tissues has become crucial for clinical and preclinical research. Here we describe an optimized method that allows the obtention of single-cell suspensions from the skin and lungs to analyze and quantify populations of tissue-resident memory CD8+ T cells by multi-parametric flow cytometry.
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