Neutrophil autophagy and extracellular DNA traps contribute to airway inflammation in severe asthma

自噬 嗜酸性粒细胞 中性粒细胞胞外陷阱 封堵器 嗜酸性阳离子蛋白 炎症 发病机制 免疫印迹 免疫学 哮喘 细胞外 生物 化学 细胞生物学 紧密连接 生物化学 细胞凋亡 基因
作者
Duy Le Pham,Ga‐Young Ban,Seung‐Hyun Kim,Yoo Seob Shin,Y.‐M. Ye,Yong‐Joon Chwae,Hae‐Sim Park
出处
期刊:Clinical & Experimental Allergy [Wiley]
卷期号:47 (1): 57-70 被引量:153
标识
DOI:10.1111/cea.12859
摘要

Summary Background Autophagy and neutrophil extracellular DNA traps ( NET s) are implicated in asthma; however, their roles in asthma pathogenesis have not been elucidated. Objectives We compared autophagy and NET production levels from peripheral blood neutrophils ( PBN s) of patients with severe asthma ( SA ) and non‐severe asthma ( NSA ). Additionally, we investigated the inflammatory effects of NET s on human airway epithelial cells ( AEC s) and peripheral blood eosinophils ( PBE s). Methods Peripheral blood neutrophils from patients with SA ( n = 30) and NSA ( n = 38) were treated with interleukin ( IL )‐8 (100 ng/mL). Autophagy (light chain 3‐ II expression) and NET production levels were evaluated by Western blot, immunofluorescence microscopy, and PicoGreen assay. The effects of NET s on AEC s were assessed by investigating cell death, cell detachment, expression of occludin and claudin‐1, and IL ‐8 production; the effects of NET s on PBE s were examined by investigating the activation and release of eosinophil cationic protein ( ECP ) and eosinophil‐derived neurotoxin ( EDN ). Results Untreated and IL ‐8‐treated PBN s from the SA group produced higher autophagy and NET levels compared with those from the NSA group ( P < 0.01). IL ‐8 increased autophagy and NET levels in PBN s from the SA group, but not from the NSA group. NET levels were correlated with autophagy levels in PBN s ( P < 0.001). IL ‐8‐induced NET production levels negatively were correlated with FEV 1/ FVC ( r = −0.700, P = 0.016). NET s induced cell death, detachment, degradation of occludin and claudin‐1, and IL ‐8 production from AEC s. Higher levels of NET ‐induced ECP and EDN were released from PBE s in SA compared with NSA groups. Conclusions and Clinical Relevance Neutrophil autophagy and NET s could enhance asthma severity by damaging airway epithelium and triggering inflammatory responses of AEC s and PBE s. Modulating neutrophil autophagy and NET production may be a new target therapy for SA .
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