鱼腥草素骨
蜘蛛丝
丝绸
融合蛋白
再生(生物学)
生物材料
化学
间充质干细胞
细胞生物学
材料科学
生物医学工程
生物化学
生物
纳米技术
重组DNA
骨钙素
医学
复合材料
酶
碱性磷酸酶
基因
作者
Sílvia Gomes,Isabel B. Leonor,João F. Mano,Rui L. Reis,David L. Kaplan
出处
期刊:Soft Matter
[The Royal Society of Chemistry]
日期:2011-01-01
卷期号:7 (10): 4964-4964
被引量:49
摘要
The remarkable mechanical characteristics of the spider silk protein major ampullate spidroin protein suggest this polymer as a promising biomaterial to consider for the fabrication of scaffolds for bone regeneration. Herein, a new functionalized spider silk-bone sialoprotein fusion protein was designed, cloned, expressed, purified and the osteogenic activity studied. Bone sialoprotein (BSP) is a multi-domain protein with the ability to induce cell attachment and differentiation and the deposition of calcium phosphates (CaP). Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) was used to assess the secondary structure of the fusion protein. In vitromineralization studies demonstrated that this new fusion protein with BSP retained the ability to induce the deposition of CaP. Studies in vitro indicated that human mesenchymal stem cells had significant improvement towards osteogenic outcomes when cultivated in the presence of the new fusion proteinvs. silk alone. The present work demonstrates the potential of this new fusion protein for future applications in bone regeneration.
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