DNA测序
计算生物学
大规模并行测序
生物
基因组
杂交基因组组装
基因组
单细胞测序
DNA
霰弹枪测序
遗传学
基因
外显子组测序
突变
出处
期刊:Annual Review of Genomics and Human Genetics
[Annual Reviews]
日期:2008-09-01
卷期号:9 (1): 387-402
被引量:2181
标识
DOI:10.1146/annurev.genom.9.081307.164359
摘要
Recent scientific discoveries that resulted from the application of next-generation DNA sequencing technologies highlight the striking impact of these massively parallel platforms on genetics. These new methods have expanded previously focused readouts from a variety of DNA preparation protocols to a genome-wide scale and have fine-tuned their resolution to single base precision. The sequencing of RNA also has transitioned and now includes full-length cDNA analyses, serial analysis of gene expression (SAGE)-based methods, and noncoding RNA discovery. Next-generation sequencing has also enabled novel applications such as the sequencing of ancient DNA samples, and has substantially widened the scope of metagenomic analysis of environmentally derived samples. Taken together, an astounding potential exists for these technologies to bring enormous change in genetic and biological research and to enhance our fundamental biological knowledge.
科研通智能强力驱动
Strongly Powered by AbleSci AI