Detection of Mycobacterium tuberculosis GlcB or HspX Antigens or devR DNA Impacts the Rapid Diagnosis of Tuberculous Meningitis in Children

结核性脑膜炎 结核分枝杆菌 肺结核 医学 脑膜炎 逻辑回归 免疫学 病毒学 微生物学 生物 内科学 病理 儿科
作者
Sagarika Haldar,Naveen Sankhyan,Neera Sharma,Anjali Bansal,Vitul Jain,Vijay Gupta,Monica Juneja,Devendra Mishra,Arti Kapil,Urvashi B. Singh,Sheffali Gulati,Veena Kalra,Jaya Sivaswami Tyagi
出处
期刊:PLOS ONE [Public Library of Science]
卷期号:7 (9): e44630-e44630 被引量:48
标识
DOI:10.1371/journal.pone.0044630
摘要

Background Tuberculous meningitis (TBM) is the most common form of neurotuberculosis and the fifth most common form of extrapulmonary TB. Early diagnosis and prompt treatment are the cornerstones of effective disease management. The accurate diagnosis of TBM poses a challenge due to an extensive differential diagnosis, low bacterial load and paucity of cerebrospinal fluid (CSF) especially in children. Methodology/Principal Findings We describe the utility of ELISA and qPCR for the detection of Mycobacterium tuberculosis (M. tb) proteins (GlcB, HspX, MPT51, Ag85B and PstS1) and DNA for the rapid diagnosis of TBM. CSF filtrates (n = 532) derived from children were classified as ‘Definite’ TBM (M. tb culture positive, n = 29), ‘Probable and Possible’ TBM (n = 165) and ‘Not-TBM’ including other cases of meningitis or neurological disorders (n = 338). ROC curves were generated from ELISA and qPCR data of ‘Definite’ TBM and Non-Tuberculous infectious meningitis (NTIM) samples and cut-off values were derived to provide ≥95% specificity. devR qPCR, GlcB, HspX and PstS1 ELISAs showed 100% (88;100) sensitivity and 96–97% specificity in ‘Definite’ TBM samples. The application of these cut-offs to ‘Probable and Possible’ TBM groups yielded excellent sensitivity (98%, 94;99) and specificity (98%, 96;99) for qPCR and for GlcB, HspX and MPT51 antigen ELISAs (sensitivity 92–95% and specificity 93–96%). A test combination of qPCR with GlcB and HspX ELISAs accurately detected all TBM samples at a specificity of ∼90%. Logistic regression analysis indicated that these tests significantly added value to the currently used algorithms for TBM diagnosis. Conclusions The detection of M. tb GlcB/HspX antigens/devR DNA in CSF is likely to improve the utility of existing algorithms for TBM diagnosis and also hasten the speed of diagnosis.

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
跳跃的鹏飞完成签到 ,获得积分0
2秒前
哈基米完成签到,获得积分10
5秒前
蓝色花生豆完成签到,获得积分0
8秒前
顾矜应助科研通管家采纳,获得10
13秒前
HJJHJH应助科研通管家采纳,获得100
13秒前
HJJHJH应助科研通管家采纳,获得30
13秒前
搜集达人应助shan采纳,获得10
26秒前
科研通AI2S应助哈基米采纳,获得10
30秒前
LingMg完成签到 ,获得积分10
31秒前
31秒前
mmnn完成签到 ,获得积分10
31秒前
byron完成签到 ,获得积分10
33秒前
shan完成签到,获得积分10
39秒前
39秒前
fantasy发布了新的文献求助10
40秒前
43秒前
shan发布了新的文献求助10
44秒前
zhangfue1989完成签到 ,获得积分10
47秒前
lll发布了新的文献求助10
49秒前
sweet雪儿妞妞完成签到 ,获得积分10
49秒前
科研通AI6.3应助fantasy采纳,获得10
50秒前
51秒前
53秒前
楚楚完成签到 ,获得积分10
56秒前
haha发布了新的文献求助10
57秒前
鲁卓林完成签到,获得积分10
59秒前
彦子完成签到 ,获得积分10
1分钟前
牛黄完成签到 ,获得积分10
1分钟前
少年完成签到 ,获得积分10
1分钟前
haha完成签到,获得积分10
1分钟前
lll完成签到 ,获得积分10
1分钟前
wwwwwl完成签到 ,获得积分10
1分钟前
白华苍松发布了新的文献求助20
1分钟前
1分钟前
bigtree完成签到 ,获得积分10
1分钟前
lilac完成签到,获得积分10
1分钟前
852应助David采纳,获得10
1分钟前
唠叨的天亦完成签到 ,获得积分10
1分钟前
Ray完成签到 ,获得积分10
1分钟前
高分求助中
(应助此贴封号)【重要!!请各用户(尤其是新用户)详细阅读】【科研通的精品贴汇总】 10000
Modern Epidemiology, Fourth Edition 5000
Handbook of pharmaceutical excipients, Ninth edition 5000
Digital Twins of Advanced Materials Processing 2000
Weaponeering, Fourth Edition – Two Volume SET 2000
Polymorphism and polytypism in crystals 1000
Social Cognition: Understanding People and Events 800
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 纳米技术 有机化学 物理 生物化学 化学工程 计算机科学 复合材料 内科学 催化作用 光电子学 物理化学 电极 冶金 遗传学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 6028342
求助须知:如何正确求助?哪些是违规求助? 7689068
关于积分的说明 16186417
捐赠科研通 5175543
什么是DOI,文献DOI怎么找? 2769540
邀请新用户注册赠送积分活动 1752998
关于科研通互助平台的介绍 1638784