造血
生物
原癌基因蛋白质c-kit
干细胞
骨髓
干细胞因子
人口
细胞生物学
免疫学
祖细胞
造血干细胞
川地34
边居
癌症研究
白细胞介素3
分子生物学
巨核细胞
胚胎干细胞
细胞分化
成体干细胞
癌症干细胞
人口学
社会学
作者
Yoshikazu Matsuoka,Yutaka Sasaki,Ryusuke Nakatsuka,Masaya Takahashi,Ryuji Iwaki,Yukari Uemura,Yoshiaki Sonoda
出处
期刊:Stem Cells
[Wiley]
日期:2011-10-25
卷期号:29 (11): 1783-1791
被引量:32
摘要
Abstract Although c-kit is expressed highly on murine hematopoietic stem cells (HSCs) and essential for bone marrow (BM) hematopoiesis, the significance of the high level of expression of c-kit on HSCs was not well determined. We show here that CD150+CD48−Lineage−Sca-1+c-kit+ HSCs in adult BM are distributed within the range of roughly a 20-fold difference in the expression level of c-kit, and that c-kit density correlates with the cycling status of the HSC population. This predisposition is more evident in the BM of mice older than 30 weeks. The HSCs in G0 phase express a lower level of c-kit both on the cell surface and inside the cells, which cannot be explained by ligand receptor binding and internalization. It is more likely that the low level of c-kit expression is a unique property of HSCs in G0. Despite functional differences in the c-kit gradient, the HSCs are uniformly hypoxic and accessible to blood perfusion. Therefore, our data indicate the possibility that the hypoxic state of the HSCs is actively regulated, rather than them being passively hypoxic through a simple anatomical isolation from the circulation.
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