Response of simultaneous sulfide and nitrate removal process on acute toxicity of substrate concentration and salinity: Single toxicity and combined toxicity

Simultaneous sulfide and nitrate removal process has performed excellent to treat nitrogen and sulfur pollutants in wastewater treatment. A high salinity stress poses a great challenge to the treatment of highly saline wastewater containing nitrate and sulfide. In addition, sulfide and nitrates are also toxic for the process, and their high concentration would inhibit the process. Therefore, the current work explores the single acute toxic effect and combined toxic effect of salinity and substrate concentration on the performance of the process from the perspective of toxicology. Considering sulfide and nitrate removal performance as an indicator, the IC50 values of sulfide were 293.20 mg S/L and 572.30 mg S/L, respectively; while those of salinity were 6.14% wt (91.78 mS/cm) and 6.63% wt (98.73 mS/cm), respectively. High substrate concentration or salinity resulted in elemental sulfur generation. The molar ratio of generated elemental sulfur to consumed sulfide(R-Sulfate) was close to 1. The response of nitrate reduction product to the elevating substrate concentration was not obvious, while its response to increasing salinity was on the contrary. With the increasing salinity (1.2% wt to 9.6% wt), molar ratio of generated nitrogen gas to consumed nitrate (R-Nitrogen gas) increased from 0.58 to 1, while molar ratio of generated nitrite to consumed nitrate (R-Nitrite) decreased from 0.43 to 0. Factorial analysis test revealed that the combined acute toxicity of substrate and salinity on sulfide oxidization and nitrate reduction were both antagonistic effects.