哈卡特
肽
生物信息学
细胞毒性
视网膜母细胞瘤蛋白
分子生物学
肽序列
细胞周期
细胞培养
肽库
体外
活力测定
对接(动物)
生物
细胞
化学
生物化学
遗传学
医学
基因
护理部
作者
Shatrah Othman,Wan Chein Tan,See Khai Lim,Nurshamimi Nor Rashid,Choon Han Heh
出处
期刊:Current Computer - Aided Drug Design
[Bentham Science]
日期:2022-05-11
卷期号:18 (4): 318-325
被引量:1
标识
DOI:10.2174/1573409918666220509214449
摘要
Human papillomavirus (HPV) is a small, non-enveloped double-stranded circular DNA virus. The high-risk types of HPV are claimed to be responsible for over 99% of cervical cancers. One of the essential HPV oncoproteins, E7, is responsible for escaping from G1/S cell cycle arrest in HPV-infected cells by binding to the retinoblastoma protein (pRb) through its LXCXE binding site.To design a peptide inhibitor targeting HPV E7 through an in silico approach.In this study, the LXCXE binding domain of pRb is used as a target to design peptide inhibitors using a reverse structure-based approach. The designed amino acid sequence from the B pocket of pRb, named peptide Y, was further investigated in vitro analysis. The cytotoxicity of the peptide was analysed in two cell lines, namely, CaSki, containing an integrated HPV16 genome, and HaCaT, an immortalized keratinocyte cell. Cell cycle analysis was also carried out in both cell lines treated with peptides.In the in silico approach, a 9-amino acids peptide sequence formed 4 conventional hydrogen bonds with LXCXE motif was selected for in vitro assay. Based on the cytotoxicity analysis, the peptide showed low toxicity in both cell lines, where the cell viability remained over 74% when treated with peptide Y. The peptide also caused an accumulation of cells in G0/G1 (+5.4%) and S phase (+10.2%) and a reduction of cells in the G2/M phase (-14.9%) in the CaSki cells with no significant effect on normal cells, indicating it is a potential HPV inhibitor.A peptide inhibitor, peptide Y, that was designed from the LXCXE binding motif in pRb can inhibit HPV E7 by causing a cell accumulation effect in G0/G1, and S phases of the cell cycle in the HPV transformed cell lines. These findings could contribute to HPV E7 peptide inhibitor in the future.
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