检出限
微流控
材料科学
微流控芯片
微分脉冲伏安法
电极
适体
炸薯条
纳米探针
线性范围
纳米技术
生物医学工程
光电子学
色谱法
化学
计算机科学
循环伏安法
电化学
纳米颗粒
生物
电信
物理化学
医学
遗传学
作者
Xinyu He,Xiaoli Wang,Changrong Ge,Shunbo Li,Li Wang,Yanhua Xu
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2022-04-01
卷期号:7 (4): 1019-1026
被引量:10
标识
DOI:10.1021/acssensors.1c02641
摘要
For the rapid and sensitive detection of vascular endothelial growth factor 165 (VEGF165) in clinical blood samples, a microfluidic sensing chip that integrates a centrifugal separation pretreatment unit and a composite nanosensing film was proposed in this paper. An efficient sensing strategy and method was established. The blood sample was first separated and extracted by centrifugal force on the centrifugal microfluidic chip within 5 min after injection. The separated plasma can be automatically transferred through the designed microchannels to the detection area integrated electrodes for subsequent differential pulse voltammetric detection. The Au NPs/MCH/Apt2 sensing film was constructed on the surface of the Au working electrode. A sandwich sensing strategy based on "double aptamers" and "nanoprobe" for VEGF165 detection was established, by which the synthetic Apt1/PThi/Au NP nanoprobe was applied to capture VEGF165 in plasma and bind to the sensing film. By this method, the detection limit of VEGF165 in whole blood was 0.67 pg/mL and the linear range was between 1 pg and 10 ng, which met the needs of clinical VEGF165 detection. It was illustrated that the proposed methodology based on the centrifugal microfluidic chip had potential application prospects in the development of the point-of-care testing fields.
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