Accurately selected 1,3,4-thiadiazole and coumarin unit to construct fluorescent probes that effectively detect 2,4,6-trinitrophenol

Two remarkablely fluorimetric probes were developed to rapidly detect 2,4,6-trinitrophenol (TNP). With the help of density functional theory (DFT) calculations, we confirmed that using 1,3,4-thiadiazole skeleton as recognition group and coumarin unit as fluorophore would show excellent application prospects in terms of TNP detection. The probes LK-1 and LK-2 displayed green and orange emission with fluorescence quenching yield as high as 83.7% and 75.1% in solution. Further evaluation demonstrated that they display outstanding selectivity and sensitivity for rapid and visual detection of TNP. Both fluorescent color and fluorescence emission spectrum had significant changes and these phenomena could easily observe via naked-eye and analytical instrument. The detection limits of them were 97 nM and 71 nM. What's more, application in real water samples and solid phase paper tests illustrated the practical significance of detection of TNP.