Highly Efficient Electrogenerated Chemiluminescence Quenching on Lipid-Coated Multifunctional Magnetic Nanoparticles for the Determination of Proteases

化学 猝灭(荧光) 化学发光 电子转移 联吡啶 光化学 纳米颗粒 荧光 结晶学 有机化学 催化作用 纳米技术 物理 晶体结构 材料科学 量子力学
作者
Xiaolin Yang,Yuxi Wei,Zimei Wang,Junxia Wang,Honglan Qi,Qiang Gao,Chengxiao Zhang
出处
期刊:Analytical Chemistry [American Chemical Society]
卷期号:94 (4): 2305-2312 被引量:18
标识
DOI:10.1021/acs.analchem.1c05033
摘要

This work reports a highly efficient electrogenerated chemiluminescence (ECL) quenching on lipid-coated multifunctional magnetic nanoparticles (MMNP) for the determination of proteases incorporating membrane-confined quenching with a specific cleavage reaction for the first time. A new ruthenium complex [Ru(bpy)2(ddcbpy)](PF6)2 (bpy = 2,2′-bipyridine, ddcbpy = 4,4′-didodecyl-carbonyl-2,2′-bipyridine with two hydrophobic long alkyl chains) was synthesized as a signal probe, while [cholesterol-(CH2)6-HSSKLQK(peptide)-ferrocene (quencher)] was designed as a specific peptide-quencher probe. The MMNP were prepared by inserting both the signal probe and the peptide-quencher probe into the cholesterol–phospholipid-coated Fe3O4 magnetic nanoparticles (Fe3O4 NP, ∼200 nm). When prostate specific antigen (PSA) taken as a model analyte was introduced into the suspension of MMNP, PSA cleaved the amide bond of SK in cholesterol-(CH2)6-HSSKLQK-Fc, and then the cleaved peptide-motif-Fc-quencher was deviated from the MMNP, resulting in the increase in the ECL intensity. It was found that the ECL quenching constant of [Ru(bpy)2(ddcbpy)]2+ on MMNP (KSV, NP/lipECL =2.68 × 107 M–1) is 137-folds higher than that on the lipid-coated electrode (KSV, lipECL=1.95 × 105 M–1) and 391-folds higher than that in the solution (KSV, aqECL =6.86 × 104 M–1). The ECL emission of Ru(bpy)32+ derivative-attached Fe3O4 NP was observed at ∼1.2 V, involving the tunnel-electron transfer pathway (TPA• + Ru(bpy)33+ = Ru(bpy)32+*). Based on the highly efficient ECL quenching of the ruthenium complex by ferrocene on the MMNP, a new ECL method was developed for PSA with a linear range from 0.01 to 1.0 ng/mL and a limit of detection of 3.0 pg/mL. This work demonstrates that the approach of ECL quenching by ferrocene on lipid-coated Fe3O4 NP is promising and could be easily extended to determine other proteases.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
云舒发布了新的文献求助10
刚刚
WYJ发布了新的文献求助10
刚刚
传奇3应助科研通管家采纳,获得10
刚刚
Hello应助科研通管家采纳,获得10
1秒前
爆米花应助科研通管家采纳,获得10
1秒前
芝士球球应助科研通管家采纳,获得10
1秒前
思源应助科研通管家采纳,获得10
1秒前
星辰大海应助科研通管家采纳,获得10
1秒前
田様应助柠檬采纳,获得10
1秒前
CodeCraft应助科研通管家采纳,获得10
1秒前
芙蓉应助科研通管家采纳,获得10
1秒前
1秒前
1秒前
大模型应助科研通管家采纳,获得10
1秒前
1秒前
劲秉应助科研通管家采纳,获得20
1秒前
1秒前
JavedAli完成签到,获得积分10
2秒前
刘艺珍完成签到,获得积分10
2秒前
郑思雨发布了新的文献求助10
3秒前
3秒前
水星逃逸发布了新的文献求助10
6秒前
7秒前
海棠花完成签到,获得积分20
8秒前
研友_VZG7GZ应助kk采纳,获得10
9秒前
开心白卉完成签到,获得积分10
9秒前
wzy5508完成签到 ,获得积分10
9秒前
9秒前
11秒前
dew发布了新的文献求助10
11秒前
NexusExplorer应助WYJ采纳,获得10
11秒前
12秒前
田野发布了新的文献求助10
13秒前
vousme完成签到 ,获得积分10
13秒前
14秒前
游尘2001完成签到,获得积分10
15秒前
16秒前
徐徐图之发布了新的文献求助10
17秒前
太阳博士完成签到,获得积分10
18秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
ALA生合成不全マウスでの糖代謝異常の分子機構解析 520
安全防范技术与工程 500
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
2-Acetyl-1-pyrroline: an important aroma component of cooked rice 500
A real-time energy management strategy based on fuzzy control and ECMS for PHEVs 400
2024 Medicinal Chemistry Reviews 400
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3191042
求助须知:如何正确求助?哪些是违规求助? 2840331
关于积分的说明 8028046
捐赠科研通 2503676
什么是DOI,文献DOI怎么找? 1337061
科研通“疑难数据库(出版商)”最低求助积分说明 638014
邀请新用户注册赠送积分活动 606459