Highly Efficient Electrogenerated Chemiluminescence Quenching on Lipid-Coated Multifunctional Magnetic Nanoparticles for the Determination of Proteases

化学 猝灭(荧光) 化学发光 电子转移 联吡啶 光化学 纳米颗粒 荧光 结晶学 有机化学 催化作用 纳米技术 物理 晶体结构 材料科学 量子力学
作者
Xiaolin Yang,Yuxi Wei,Zimei Wang,Junxia Wang,Honglan Qi,Qiang Gao,Chengxiao Zhang
出处
期刊:Analytical Chemistry [American Chemical Society]
卷期号:94 (4): 2305-2312 被引量:18
标识
DOI:10.1021/acs.analchem.1c05033
摘要

This work reports a highly efficient electrogenerated chemiluminescence (ECL) quenching on lipid-coated multifunctional magnetic nanoparticles (MMNP) for the determination of proteases incorporating membrane-confined quenching with a specific cleavage reaction for the first time. A new ruthenium complex [Ru(bpy)2(ddcbpy)](PF6)2 (bpy = 2,2′-bipyridine, ddcbpy = 4,4′-didodecyl-carbonyl-2,2′-bipyridine with two hydrophobic long alkyl chains) was synthesized as a signal probe, while [cholesterol-(CH2)6-HSSKLQK(peptide)-ferrocene (quencher)] was designed as a specific peptide-quencher probe. The MMNP were prepared by inserting both the signal probe and the peptide-quencher probe into the cholesterol–phospholipid-coated Fe3O4 magnetic nanoparticles (Fe3O4 NP, ∼200 nm). When prostate specific antigen (PSA) taken as a model analyte was introduced into the suspension of MMNP, PSA cleaved the amide bond of SK in cholesterol-(CH2)6-HSSKLQK-Fc, and then the cleaved peptide-motif-Fc-quencher was deviated from the MMNP, resulting in the increase in the ECL intensity. It was found that the ECL quenching constant of [Ru(bpy)2(ddcbpy)]2+ on MMNP (KSV, NP/lipECL =2.68 × 107 M–1) is 137-folds higher than that on the lipid-coated electrode (KSV, lipECL=1.95 × 105 M–1) and 391-folds higher than that in the solution (KSV, aqECL =6.86 × 104 M–1). The ECL emission of Ru(bpy)32+ derivative-attached Fe3O4 NP was observed at ∼1.2 V, involving the tunnel-electron transfer pathway (TPA• + Ru(bpy)33+ = Ru(bpy)32+*). Based on the highly efficient ECL quenching of the ruthenium complex by ferrocene on the MMNP, a new ECL method was developed for PSA with a linear range from 0.01 to 1.0 ng/mL and a limit of detection of 3.0 pg/mL. This work demonstrates that the approach of ECL quenching by ferrocene on lipid-coated Fe3O4 NP is promising and could be easily extended to determine other proteases.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
天天快乐应助可可西里采纳,获得10
刚刚
NIUBEN发布了新的文献求助10
1秒前
yaoccccchen发布了新的文献求助10
3秒前
鹤鸣发布了新的文献求助30
3秒前
hyx发布了新的文献求助10
3秒前
4秒前
石夜一觞完成签到,获得积分10
6秒前
无花果应助Phoebe Li采纳,获得10
6秒前
6秒前
蟒玉朝天完成签到 ,获得积分10
8秒前
活泼新儿完成签到 ,获得积分10
8秒前
8秒前
CodeCraft应助zrw采纳,获得10
8秒前
9秒前
随机子应助wxy采纳,获得10
9秒前
啊呜完成签到,获得积分10
10秒前
11秒前
12秒前
111111111发布了新的文献求助10
13秒前
14秒前
wanci应助yaoccccchen采纳,获得10
14秒前
啊啊啊完成签到 ,获得积分10
15秒前
15秒前
秋风暖暖应助受伤南霜采纳,获得10
15秒前
随机子应助tuntunliu采纳,获得10
15秒前
xiaoxiao发布了新的文献求助30
16秒前
小思怡完成签到,获得积分10
16秒前
可可西里发布了新的文献求助10
16秒前
不回首发布了新的文献求助10
16秒前
17秒前
17秒前
所所应助乐山乐水采纳,获得10
18秒前
18秒前
1908发布了新的文献求助10
18秒前
摆烂fish发布了新的文献求助10
18秒前
英俊的铭应助要减肥白云采纳,获得10
19秒前
19秒前
19秒前
研友_VZG7GZ应助yyy采纳,获得10
19秒前
所所应助张晟晟采纳,获得30
19秒前
高分求助中
Evolution 3rd edition 1500
保险藏宝图 1000
Lire en communiste 1000
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 700
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 700
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
2-Acetyl-1-pyrroline: an important aroma component of cooked rice 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3181539
求助须知:如何正确求助?哪些是违规求助? 2831784
关于积分的说明 7986720
捐赠科研通 2493805
什么是DOI,文献DOI怎么找? 1330348
科研通“疑难数据库(出版商)”最低求助积分说明 635955
版权声明 602955