亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Highly Efficient Electrogenerated Chemiluminescence Quenching on Lipid-Coated Multifunctional Magnetic Nanoparticles for the Determination of Proteases

化学 猝灭(荧光) 化学发光 电子转移 联吡啶 光化学 纳米颗粒 荧光 结晶学 有机化学 催化作用 纳米技术 物理 晶体结构 材料科学 量子力学
作者
Xiaolin Yang,Yuxi Wei,Zimei Wang,Junxia Wang,Honglan Qi,Qiang Gao,Chengxiao Zhang
出处
期刊:Analytical Chemistry [American Chemical Society]
卷期号:94 (4): 2305-2312 被引量:18
标识
DOI:10.1021/acs.analchem.1c05033
摘要

This work reports a highly efficient electrogenerated chemiluminescence (ECL) quenching on lipid-coated multifunctional magnetic nanoparticles (MMNP) for the determination of proteases incorporating membrane-confined quenching with a specific cleavage reaction for the first time. A new ruthenium complex [Ru(bpy)2(ddcbpy)](PF6)2 (bpy = 2,2′-bipyridine, ddcbpy = 4,4′-didodecyl-carbonyl-2,2′-bipyridine with two hydrophobic long alkyl chains) was synthesized as a signal probe, while [cholesterol-(CH2)6-HSSKLQK(peptide)-ferrocene (quencher)] was designed as a specific peptide-quencher probe. The MMNP were prepared by inserting both the signal probe and the peptide-quencher probe into the cholesterol–phospholipid-coated Fe3O4 magnetic nanoparticles (Fe3O4 NP, ∼200 nm). When prostate specific antigen (PSA) taken as a model analyte was introduced into the suspension of MMNP, PSA cleaved the amide bond of SK in cholesterol-(CH2)6-HSSKLQK-Fc, and then the cleaved peptide-motif-Fc-quencher was deviated from the MMNP, resulting in the increase in the ECL intensity. It was found that the ECL quenching constant of [Ru(bpy)2(ddcbpy)]2+ on MMNP (KSV, NP/lipECL =2.68 × 107 M–1) is 137-folds higher than that on the lipid-coated electrode (KSV, lipECL=1.95 × 105 M–1) and 391-folds higher than that in the solution (KSV, aqECL =6.86 × 104 M–1). The ECL emission of Ru(bpy)32+ derivative-attached Fe3O4 NP was observed at ∼1.2 V, involving the tunnel-electron transfer pathway (TPA• + Ru(bpy)33+ = Ru(bpy)32+*). Based on the highly efficient ECL quenching of the ruthenium complex by ferrocene on the MMNP, a new ECL method was developed for PSA with a linear range from 0.01 to 1.0 ng/mL and a limit of detection of 3.0 pg/mL. This work demonstrates that the approach of ECL quenching by ferrocene on lipid-coated Fe3O4 NP is promising and could be easily extended to determine other proteases.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
觅雪格完成签到 ,获得积分10
12秒前
通科研完成签到 ,获得积分10
54秒前
1分钟前
1分钟前
1分钟前
CGBY完成签到 ,获得积分10
1分钟前
2分钟前
2分钟前
3分钟前
3分钟前
3分钟前
杨同学发布了新的文献求助10
3分钟前
4分钟前
甜蜜发带完成签到 ,获得积分10
4分钟前
yyr完成签到 ,获得积分20
4分钟前
4分钟前
4分钟前
葛力完成签到,获得积分10
4分钟前
5分钟前
5分钟前
执着的飞瑶完成签到,获得积分20
5分钟前
毛豆应助司徒无剑采纳,获得20
5分钟前
5分钟前
5分钟前
6分钟前
6分钟前
赘婿应助Sandy采纳,获得10
6分钟前
7分钟前
刻苦羽毛完成签到 ,获得积分10
7分钟前
7分钟前
7分钟前
8分钟前
8分钟前
8分钟前
8分钟前
CQU科研萌新完成签到,获得积分10
8分钟前
Echo完成签到,获得积分10
8分钟前
9分钟前
冬去春来完成签到 ,获得积分10
9分钟前
9分钟前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
Aspects of Babylonian celestial divination: the lunar eclipse tablets of Enūma Anu Enlil 500
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
Dictionary of socialism 350
Mixed-anion Compounds 300
Geochemistry, 2nd Edition 地球化学经典教科书第二版 300
Idoxuridine 260
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3196776
求助须知:如何正确求助?哪些是违规求助? 2845469
关于积分的说明 8054183
捐赠科研通 2510065
什么是DOI,文献DOI怎么找? 1342246
科研通“疑难数据库(出版商)”最低求助积分说明 639340
邀请新用户注册赠送积分活动 608642