Highly Efficient Electrogenerated Chemiluminescence Quenching on Lipid-Coated Multifunctional Magnetic Nanoparticles for the Determination of Proteases

化学 猝灭(荧光) 化学发光 电子转移 联吡啶 光化学 纳米颗粒 荧光 结晶学 有机化学 催化作用 纳米技术 物理 晶体结构 材料科学 量子力学
作者
Xiaolin Yang,Yuxi Wei,Zimei Wang,Junxia Wang,Honglan Qi,Qiang Gao,Chengxiao Zhang
出处
期刊:Analytical Chemistry [American Chemical Society]
卷期号:94 (4): 2305-2312 被引量:18
标识
DOI:10.1021/acs.analchem.1c05033
摘要

This work reports a highly efficient electrogenerated chemiluminescence (ECL) quenching on lipid-coated multifunctional magnetic nanoparticles (MMNP) for the determination of proteases incorporating membrane-confined quenching with a specific cleavage reaction for the first time. A new ruthenium complex [Ru(bpy)2(ddcbpy)](PF6)2 (bpy = 2,2′-bipyridine, ddcbpy = 4,4′-didodecyl-carbonyl-2,2′-bipyridine with two hydrophobic long alkyl chains) was synthesized as a signal probe, while [cholesterol-(CH2)6-HSSKLQK(peptide)-ferrocene (quencher)] was designed as a specific peptide-quencher probe. The MMNP were prepared by inserting both the signal probe and the peptide-quencher probe into the cholesterol–phospholipid-coated Fe3O4 magnetic nanoparticles (Fe3O4 NP, ∼200 nm). When prostate specific antigen (PSA) taken as a model analyte was introduced into the suspension of MMNP, PSA cleaved the amide bond of SK in cholesterol-(CH2)6-HSSKLQK-Fc, and then the cleaved peptide-motif-Fc-quencher was deviated from the MMNP, resulting in the increase in the ECL intensity. It was found that the ECL quenching constant of [Ru(bpy)2(ddcbpy)]2+ on MMNP (KSV, NP/lipECL =2.68 × 107 M–1) is 137-folds higher than that on the lipid-coated electrode (KSV, lipECL=1.95 × 105 M–1) and 391-folds higher than that in the solution (KSV, aqECL =6.86 × 104 M–1). The ECL emission of Ru(bpy)32+ derivative-attached Fe3O4 NP was observed at ∼1.2 V, involving the tunnel-electron transfer pathway (TPA• + Ru(bpy)33+ = Ru(bpy)32+*). Based on the highly efficient ECL quenching of the ruthenium complex by ferrocene on the MMNP, a new ECL method was developed for PSA with a linear range from 0.01 to 1.0 ng/mL and a limit of detection of 3.0 pg/mL. This work demonstrates that the approach of ECL quenching by ferrocene on lipid-coated Fe3O4 NP is promising and could be easily extended to determine other proteases.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
木子木子粒完成签到 ,获得积分10
1秒前
asir完成签到,获得积分10
1秒前
小吴搞科研完成签到,获得积分10
2秒前
2秒前
4秒前
高兴的店员完成签到,获得积分10
5秒前
卓涛完成签到,获得积分10
6秒前
6秒前
8秒前
9秒前
lll完成签到 ,获得积分10
10秒前
11秒前
12秒前
一定要早睡完成签到,获得积分10
13秒前
研究牲完成签到,获得积分10
14秒前
小郭完成签到,获得积分10
14秒前
15秒前
8R60d8应助一个橡果采纳,获得10
15秒前
16秒前
培培完成签到 ,获得积分10
16秒前
哈哈哈哈完成签到 ,获得积分10
17秒前
17秒前
鱼鱼片片发布了新的文献求助10
18秒前
18秒前
LYL发布了新的文献求助10
19秒前
19秒前
20秒前
20秒前
er发布了新的文献求助10
20秒前
斯文败类应助学不懂采纳,获得30
21秒前
穿裤子的发糕完成签到,获得积分20
21秒前
毛豆应助小郭采纳,获得10
22秒前
23秒前
Ling发布了新的文献求助10
23秒前
24秒前
Rita发布了新的文献求助10
24秒前
1234发布了新的文献求助10
24秒前
丘比特应助阔达的棒棒糖采纳,获得10
25秒前
25秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
Write Like a Chemist: A Guide and Resource (第二版) 600
Mixed-anion Compounds 600
ALA生合成不全マウスでの糖代謝異常の分子機構解析 520
Earth System Geophysics 500
Aspects of Babylonian celestial divination: the lunar eclipse tablets of Enūma Anu Enlil 500
Geochemistry, 2nd Edition 地球化学经典教科书第二版 401
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3199568
求助须知:如何正确求助?哪些是违规求助? 2848333
关于积分的说明 8067643
捐赠科研通 2513090
什么是DOI,文献DOI怎么找? 1345419
科研通“疑难数据库(出版商)”最低求助积分说明 640058
邀请新用户注册赠送积分活动 609751