化学
色谱法
橙皮素
葡萄糖醛酸
柚皮素
化学成分
质谱法
黄酮类
代谢物
糖苷
类黄酮
立体化学
有机化学
抗氧化剂
生物化学
作者
Chao Ma,Wenyuan Gao,Ying Gao,Shuli Man,Luqi Huang,Changxiao Liu
摘要
Fructus Aurantii, as a component of several compound formulae, shows many bioactivities. As is well known, the constituents of traditional Chinese medicines are very complex and multiple constituents are responsible for the therapeutic effect. However, the concrete compounds are difficult to confirm. Therefore, studies on the constituents absorbed into serum and their metabolites are necessary.To search for the active constituents in Fructus Aurantii.An ultra-performance liquid chromatography-photodiode array detector-quadrupole time-of-flight mass spectrometry (UPLC-PDA-Q-TOF/MS) method was established to analyse the aqueous extract in Fructus Aurantii and the constituents absorbed into blood. Compound identification was made by matching the empirical molecular formula with those of the reported compounds and UV spectra, and further elucidated by lower energy CID mass spectra.Twelve flavonoid O-glycosides were detected, and nine compounds were tentatively identified as polymethoxylated flavones. Six parent compounds were identified and four metabolites were observed in rat plasma, two of which were identified as naringenin glucuronide and hesperetin glucuronide, respectively.The approach developed has proved useful in the study of the active constituents in traditional Chinese medicines.
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