First Report of Natural Infection of Siraitia grosvenorii by Cucumber Green Mottle Mosaic Virus in China

HomePlant DiseaseVol. 104, No. 4First Report of Natural Infection of Siraitia grosvenorii by Cucumber Green Mottle Mosaic Virus in China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Natural Infection of Siraitia grosvenorii by Cucumber Green Mottle Mosaic Virus in ChinaHuiting Xie, Bixia Qin, Lixian Cui, Zhanbiao Li, Jinqing Chen, Yongcai Huang, and Jianhe CaiHuiting Xiehttp://orcid.org/0000-0003-4424-2805Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Plant Protection Research Institute, Guangxi Academy of Agricultural Sciences, Guangxi, Nanning, 530007, P.R. ChinaSearch for more papers by this author, Bixia Qin†Corresponding authors: B. Qin; E-mail Address: qinbx@126.com and J. Cai; E-mail Address: caijianhe@gxaas.netGuangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Plant Protection Research Institute, Guangxi Academy of Agricultural Sciences, Guangxi, Nanning, 530007, P.R. ChinaSearch for more papers by this author, Lixian CuiGuangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Plant Protection Research Institute, Guangxi Academy of Agricultural Sciences, Guangxi, Nanning, 530007, P.R. ChinaSearch for more papers by this author, Zhanbiao Lihttp://orcid.org/0000-0001-8331-4168Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Plant Protection Research Institute, Guangxi Academy of Agricultural Sciences, Guangxi, Nanning, 530007, P.R. ChinaSearch for more papers by this author, Jinqing ChenGuangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Plant Protection Research Institute, Guangxi Academy of Agricultural Sciences, Guangxi, Nanning, 530007, P.R. ChinaSearch for more papers by this author, Yongcai HuangBiotechnology Research Institute, Guangxi Academy of Agricultural Sciences, Guangxi, Nanning, 530007, P.R. ChinaSearch for more papers by this author, and Jianhe Cai†Corresponding authors: B. Qin; E-mail Address: qinbx@126.com and J. Cai; E-mail Address: caijianhe@gxaas.netGuangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Plant Protection Research Institute, Guangxi Academy of Agricultural Sciences, Guangxi, Nanning, 530007, P.R. ChinaSearch for more papers by this author AffiliationsAuthors and Affiliations Huiting Xie1 Bixia Qin1 † Lixian Cui1 Zhanbiao Li1 Jinqing Chen1 Yongcai Huang2 Jianhe Cai1 † 1Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Plant Protection Research Institute, Guangxi Academy of Agricultural Sciences, Guangxi, Nanning, 530007, P.R. China 2Biotechnology Research Institute, Guangxi Academy of Agricultural Sciences, Guangxi, Nanning, 530007, P.R. China Published Online:10 Feb 2020https://doi.org/10.1094/PDIS-07-19-1354-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Fruit of Siraitia grosvenorii (family Cucurbitaceae) are used as a sweetener in beverage and traditional herbal medicine production in China. Some viruses have been known to infect S. grosvenorii, such as papaya ringspot virus (PRSV), watermelon mosaic virus (WMV), and zucchini yellow mosaic virus (ZYMV) (Cai et al. 2001; Liao et al. 2005; Qin et al. 2005). In August 2017, six S. grosvenorii samples (nos. LHG-1 to LHG-6) that showed mosaic, chlorosis, and mottling of leaves were collected from Yongfu, Guangxi province, China. All symptomatic samples were tested by enzyme-linked immunosorbent assay (ELISA) in double antibody sandwich format for 10 known cucurbit-infecting viruses, including cucumber green mottle mosaic virus (CGMMV), cucumber mosaic virus, kyuri green mottle mosaic virus, PRSV, squash mosaic virus, tobacco mosaic virus (TMV), watermelon silver mottle virus, WMV, zucchini green mottle mosaic virus, and ZYMV. The sample LHG-1 with chlorosis and mottle symptoms reacted positively against the antisera of CGMMV and TMV (Adgen, U.K.) but negatively against antisera of the other viruses. The other samples were negative in ELISA for all viruses tested. Total RNA was extracted from the LHG-1 sample using TRIzol reagent (Takara Dalian, China) following the manufacturer’s instructions. Reverse transcription polymerase chain reaction (RT-PCR) assays using CGMMV-specific primer pair CGM1/CGM2 (Huang et al. 2007) and TMV-specific primers (Li et al. 2000) showed a positive RT-PCR product of the expected size (650 bp) for CGMMV but not for TMV. The amplicon was further cloned into pMD-18-T vector (Takara Dalian) and sequenced (GenBank accession no. MK858182). Sequence analysis revealed that the RT-PCR product showed 99% nucleotide identity to the sequences of CGMMV isolates available in GenBank (accession nos. HM008919, KY753928, KX883833, KP868654, KM873786, and MH271443). The virus in LHG-1 was therefore identified as an isolate of CGMMV and named as CGMMV-LHG. Additionally, to confirm the infectivity of CGMMV-LHG, leaf sap extract in phosphate buffer (0.1 M, pH 7.0) of LHG-1 was mechanically inoculated on leaves of S. grosvenorii and Lagenaria siceraria seedlings. Ten days after inoculation, S. grosvenorii produced symptoms similar to the diseased plants in the field, and systemic mosaic and vein-banding were observed on L. siceraria. The control seedlings inoculated with phosphate buffer remained symptomless. Presence of CGMMV in inoculated plants was confirmed by RT-PCR. To the best of our knowledge, this is the first report of CGMMV infection on S. grosvenorii under natural conditions. The virus can be transmitted by seeds, infects various cucurbit species, and causes yield losses all over the world (Liu et al. 2014; Wu et al. 2011). Use of virus-free seeds and seedlings will be helpful to control CGMMV in S. grosvenorii production areas.The author(s) declare no conflict of interest.References:Cai, J. H., et al. 2001. J. Guangxi Sci. 8:66. ISI, Google ScholarHuang, J., et al. 2007. Chin. Agric. Sci. Bull. (Beijing) 23:319. Google ScholarLi, F., et al. 2000. J. Zhejiang Univ. 26:261. Google ScholarLiao, Y. M., et al. 2005. Plant Dis. 89:530. https://doi.org/10.1094/PD-89-0530A Link, ISI, Google ScholarLiu, H. W., et al. 2014. Plant Pathol. 63:72. https://doi.org/10.1111/ppa.12065 Crossref, ISI, Google ScholarQin, B. X., et al. 2005. Chin. J. Virol. 6:468. Google ScholarWu, H. J., et al. 2011. Sci. Agric. Sin. 44:1527. https://doi.org/10.3864/j.issn.0578-1752.2011.07.028 Google ScholarThe author(s) declare no conflict of interest.Funding: This work was supported by the Open Project Fund of Henan Provincial Key Laboratory of Fruit and Cucurbit Biology (HNS-201508-10), Open Project Fund of Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests (17-259-47-KF-2), Special Fund for Guangxi Innovation Team (Characteristic Fruit) of National System of Modern Agriculture Industrial Technology (nycytxgxcxtd-04-19-2), and Basic Research Project of Guangxi Academy of Agricultural Sciences (no. 2015YT42).DetailsFiguresLiterature CitedRelated Vol. 104, No. 4 April 2020SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionDisease symptoms of leaves of the peach cultivar Royal Bell caused by Xanthomonas arboricola pv. pruni (R. Iličić and T. Popović). Photo credit: R. Iličić. Symptoms of chilli yellow ringspot virus on a chilli pepper plant (K. Y. Zheng et al.). Photo credit: J. H. Dong. Metrics Downloaded 855 times Article History Issue Date: 3 Apr 2020Published: 10 Feb 2020First Look: 16 Dec 2019Accepted: 11 Dec 2019 Page: 1263 Information© 2020 The American Phytopathological SocietyFundingOpen Project Fund of Henan Provincial Key Laboratory of Fruit and Cucurbit BiologyGrant/Award Number: HNS-201508-10Open Project Fund of Guangxi Key Laboratory of Biology for Crop Diseases and Insect PestsGrant/Award Number: 17-259-47-KF-2Special Fund for Guangxi Innovation Team (Characteristic Fruit) of National System of Modern Agriculture Industrial TechnologyGrant/Award Number: nycytxgxcxtd-04-19-2Basic Research Project of Guangxi Academy of Agricultural SciencesGrant/Award Number: no. 2015YT42Keywordsviruses and viroidsSiraitia grosvenoriicucumber green mottle mosaic virusnatural infectionThe author(s) declare no conflict of interest.