化学
适体
催化作用
组合化学
检出限
三磷酸腺苷
纳米颗粒
纳米技术
生物物理学
生物化学
色谱法
分子生物学
生物
材料科学
作者
Shuai Li,Xiaoting Zhao,Xiaoxue Yu,Yuqi Wan,Mengyuan Yin,Wenwen Zhang,Bingqiang Cao,Hua Wang
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2019-10-17
卷期号:91 (22): 14737-14742
被引量:113
标识
DOI:10.1021/acs.analchem.9b04116
摘要
In this work, a simple and highly selective colorimetric method has been developed for quantifying trace-level ATP using Fe3O4 nanoparticles (Fe3O4 NPs). It was discovered that Fe3O4 NPs could present the dramatically enhanced catalysis once anchored with ATP-specific aptamers (Apts), which is about 6-fold larger than that of bare Fe3O4 NPs. In the presence of ATP, however, the Apts would be desorbed from Fe3O4 NPs due to the Apts-target binding event, leading to the decrease of catalysis rationally depending on ATP concentrations. A colorimetric strategy was thereby developed to facilitate the highly selective detection of ATP, showing the linear concentrations ranging from 0.50 to 100 μM. Subsequently, the developed ATP sensor was employed for the evaluation of ATP in blood with the analysis performances comparably better than those of the documented detection methods, showing the potential applications in the clinical laboratory for the detective diagnosis of some ATP-indicative diseases. Importantly, such a catalysis-based detection strategy should be extended to other kinds of nanozymes with intrinsic catalysis properties (i.e., peroxidase and oxidase-like activities), promising as a universal candidate for monitoring various biological species simply by using target-specific recognition elements like Apts and antibodies.
科研通智能强力驱动
Strongly Powered by AbleSci AI