化学
纳米颗粒
纳米材料
生物流体
血液蛋白质类
肽
体内
人口
生物物理学
纳米技术
生物化学
生物
色谱法
材料科学
生物技术
人口学
社会学
作者
Sun Young Lee,Jin Gyeong Son,Jeong Hee Moon,Sunho Joh,Tae Geol Lee
出处
期刊:Biointerphases
[American Institute of Physics]
日期:2020-11-01
卷期号:15 (6)
被引量:11
摘要
Nanomaterials form a complex called “protein corona” by contacting with protein-containing biological fluids such as serum when they are exposed to physiological environments. The characteristics of these proteins, which are one of the substantial factors in cellular response, are affected by the interactions between the nanomaterials and the biological systems. Many studies have investigated the biological behaviors of nanomaterials by conducting experiments in vitro and in vivo; however, the origin of the biological materials used is rather inconsistent. This is due to the fact that the composition of the protein coronas may differ depending on the animal origin, not on the composition or size of the nanoparticles. The resulting differences in the composition of the protein coronas can lead to different conclusions. To identify the differences in protein corona formation among sera of different species, we investigated protein coronas of gold and silica nanoparticles in serum obtained from various species. Using comparative proteomic analysis, common proteins adsorbed onto each nanoparticle among the three different sera were identified as highly abundant proteins in the serum. These findings indicate that protein corona formation is dependent on the serum population rather than the size or type of the nanoparticles. Additionally, in the physiological classification of protein coronas, human serum (HS) was found to be rich in apolipoproteins. In conclusion, our data indicate that HS components are different from those of bovine or mouse, indicating that the serum species origin should be carefully considered when selecting a biological fluid.
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