Odorant‐binding proteins and chemosensory proteins potentially involved in host plant recognition in the Asian citrus psyllid, Diaphorina citri

陈皮 生物 基因 气味结合蛋白 寄主(生物学) 基因组 遗传学 计算生物学 植物 半翅目
作者
He Zhang,Junlong Chen,Jiahao Lin,Jintian Lin,Zhongzhen Wu
出处
期刊:Pest Management Science [Wiley]
卷期号:76 (8): 2609-2618 被引量:31
标识
DOI:10.1002/ps.5799
摘要

Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are two families of small water-soluble proteins involved in odor detection and subsequent signal transmission. Determination of their binding mechanisms and specificity towards different odorants is important for developing OBPs/CSPs as targets in pest control management.We re-annotated genes encoding putative OBPs and CSPs in the Asian citrus psyllid (Diaphorina citri) draft genome using various bioinformatic tools. Genes encoding nine OBPs (seven Classic and two Plus-C) and 12 CSPs were identified, consistent with our previous transcriptomic results. Tissue-specific and developmental expression analyses suggested that genes encoding six OBPs and four CSPs were predominantly expressed in antennae, and displayed various expression patterns in different development stages, suggesting potential involvement in olfactory perception. Competitive fluorescence binding assays with 13 candidate ligands, including known host plant volatiles, sex pheromone components and repellents, showed that DcitOBP3 could bind to various odorants, whereas DcitOBP6, 8 and 9 bound specifically to host plant terpenoids. DcitCSP1 and 12 could also bind to certain terpenoids with high binding specificity.OBP- and CSP-encoding genes were systematically identified by annotating the draft D. citri genome and those potentially involved in odorant detection and signal transmission were identified by analyzing their tissue-expression profiles and odorant-binding affinities, particularly to the peripheral molecular perception of host plant terpenoids. The identified genes may provide potential targets for efficient pest control. © 2020 Society of Chemical Industry.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
狂野香菱完成签到,获得积分20
刚刚
spark810应助七分糖采纳,获得30
刚刚
IKARUTO发布了新的文献求助10
1秒前
1秒前
1秒前
2秒前
卓梨完成签到 ,获得积分10
2秒前
高兴的灵雁完成签到 ,获得积分10
2秒前
2秒前
33完成签到,获得积分10
2秒前
海阔凭发布了新的文献求助10
3秒前
4秒前
南北应助Ginelle采纳,获得10
5秒前
5秒前
丘比特应助二十一采纳,获得10
6秒前
时尚蜜蜂完成签到 ,获得积分10
6秒前
狂野香菱发布了新的文献求助30
6秒前
Chen完成签到,获得积分10
6秒前
二甲双胍发布了新的文献求助10
7秒前
英姑应助549sysfzr采纳,获得10
7秒前
terasatang完成签到,获得积分10
7秒前
无异常发布了新的文献求助10
7秒前
7秒前
伟蓓1314完成签到,获得积分10
7秒前
hahahahaha发布了新的文献求助10
7秒前
笨笨的完成签到,获得积分10
8秒前
9秒前
丘比特应助晴天采纳,获得10
9秒前
王梦瑶发布了新的文献求助10
10秒前
11秒前
tyZhang发布了新的文献求助10
12秒前
青衍应助xuan采纳,获得10
12秒前
13秒前
13秒前
13秒前
可靠的0发布了新的文献求助10
13秒前
爱学习的小王完成签到,获得积分10
15秒前
海阔凭完成签到,获得积分20
15秒前
我是雅婷发布了新的文献求助10
15秒前
高分求助中
Evolution 10000
Becoming: An Introduction to Jung's Concept of Individuation 600
Distribution Dependent Stochastic Differential Equations 500
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
A new species of Velataspis (Hemiptera Coccoidea Diaspididae) from tea in Assam 500
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 500
The Kinetic Nitration and Basicity of 1,2,4-Triazol-5-ones 440
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3159180
求助须知:如何正确求助?哪些是违规求助? 2810321
关于积分的说明 7887314
捐赠科研通 2469183
什么是DOI,文献DOI怎么找? 1314687
科研通“疑难数据库(出版商)”最低求助积分说明 630682
版权声明 602012