[Role of PPARα in the oxidative damage of rat liver cells BRL-3A induced by perfluorooctanoic acid].

全氟辛酸 兴奋剂 免疫印迹 过氧化物酶体增殖物激活受体 化学 基因表达 内分泌学 内科学 分子生物学 生物 基因 受体 生物化学 医学
作者
Li Wang,Ziyan Hu,Weiye Wang,Zhaoyan Wen,Mengying Kan,Hui Liu
出处
期刊:PubMed 卷期号:47 (3): 465-470
链接
标识
摘要

To investigate the role of PPARα in oxidative damage of BRL-3A cells induced by perfluorooctanoic acid( PFOA) by inhibiting and activating gene expression.In vitro culture of rat liver BRL-3A cells were divided into blank control group, PFOA experimental control group, PPARα inhibition group( GW6471), PPARα agonist group( WY14643), PPARα inhibitor pretreatment PFOA group( GW6471 + PFOA), PPARα agonist pretreatment PFOA group( WY14643 + PFOA). Fluorescence immunocytochemistry was used to detect the expression of PPARα. The expression of PPARα and its downstream target gene was detected by q PCR. The expression of related protein was detected by Western blot.The expression of PPARα in rat liver BRL-3A cells was successfully inhibited and stimulated by inhibitors and agonists( P < 0. 05). Compared with the blank control group and the PFOA experimental control group, there was a significant decrease in the content of ROS in the WY14643 + PFOA group compared with the blank control group and the PFOA experimental control group( P < 0. 05). The expression of PPARα and its downstream gene Cyp4a1 in GW6471 + PFOA group was higher than that in PPARα inhibitor group( P < 0. 05), but it was significantly lower than that in PFOA experimental control group( P < 0. 05). The expression of related genes in WY14643 + PFOA group was significantly lower than that in PPARα agonist group( P < 0. 05). The protein expression of PPARα in GW6471 + PFOA group was up-regulated compared with the inhibitor group, there was no difference compared with the blank control group. The protein expression of PPARα in WY14643 + PFOA group was not significantly different from that in agonist group, but it was significantly higher than that in PFOA experimental control group( P < 0. 05).PFOA exposure can activate the expression of PPARα, remove ROS, PPARα played a protective role in PFOA-induced rat liver cell oxidative damage.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
pcr163应助杨茉采纳,获得60
3秒前
6秒前
sine_mora完成签到,获得积分10
7秒前
8秒前
Hello应助fly the bike采纳,获得30
9秒前
9秒前
吞吞完成签到,获得积分10
10秒前
11秒前
一起看海发布了新的文献求助10
13秒前
14秒前
迷人的Jack发布了新的文献求助10
16秒前
zmxssg008完成签到,获得积分10
16秒前
KoitoYuu发布了新的文献求助10
18秒前
宫宛儿完成签到,获得积分10
18秒前
blance完成签到 ,获得积分10
20秒前
doudou发布了新的文献求助10
22秒前
镜子应助煎饼狗子采纳,获得10
22秒前
22秒前
23秒前
24秒前
ding应助自由的青烟采纳,获得10
28秒前
28秒前
29秒前
30秒前
31秒前
32秒前
wlz完成签到,获得积分10
32秒前
沉默烨霖发布了新的文献求助10
34秒前
38秒前
碧蓝世界完成签到 ,获得积分10
39秒前
赘婿应助Sunsky采纳,获得20
41秒前
Denny发布了新的文献求助10
43秒前
46秒前
桐桐应助缓慢的藏鸟采纳,获得10
47秒前
烩面大师完成签到 ,获得积分10
49秒前
北堂完成签到 ,获得积分10
51秒前
52秒前
53秒前
54秒前
威武的翠安完成签到 ,获得积分10
55秒前
高分求助中
The ACS Guide to Scholarly Communication 2500
Sustainability in Tides Chemistry 2000
Pharmacogenomics: Applications to Patient Care, Third Edition 1000
Studien zur Ideengeschichte der Gesetzgebung 1000
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
Threaded Harmony: A Sustainable Approach to Fashion 810
《粉体与多孔固体材料的吸附原理、方法及应用》(需要中文翻译版,化学工业出版社,陈建,周力,王奋英等译) 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3084205
求助须知:如何正确求助?哪些是违规求助? 2737236
关于积分的说明 7544149
捐赠科研通 2386784
什么是DOI,文献DOI怎么找? 1265552
科研通“疑难数据库(出版商)”最低求助积分说明 613127
版权声明 598187