细胞凋亡
卡尔帕因
凋亡诱导因子
组织蛋白酶B
线粒体
活性氧
组织蛋白酶
细胞生物学
细胞色素c
组织蛋白酶D
化学
半胱氨酸蛋白酶3
半胱氨酸蛋白酶
分子生物学
程序性细胞死亡
生物
生物化学
酶
作者
Jiaying Zhang,Guoyuan Ma,Zengjun Guo,Qunli Yu,Ling Han,Mingshan Han,Yueming Zhu
标识
DOI:10.1016/j.foodchem.2018.06.032
摘要
This study investigated the apoptosis pathway mediated by apoptosis-inducing factors (AIF) and internal factors that influence the release of AIF. The results indicated that the AIF expression was decreased in mitochondria and increased in nucleus (P < 0.05). However, no significant differences were observed in the AIF expression in mitochondria with caspase inhibitor treatment. The optical density of nucleus and mitochondrial swelling was significant increased (P < 0.05). Reactive oxygen species (ROS) fell gradually within the first 6 h and increased in the next 24 h. Calpain I activity was decreased, Ca2+ concentration, cathepsin B and D activities was increased (P < 0.05). The results demonstrated that AIF-mediated caspase-dependent pathway was a new mitochondrial apoptosis pathway and that mitochondrial swelling, ROS content, Ca2+ concentration, calpain I, cathepsin B and cathepsin D activities are the key influencing factors in apoptosis in postmortem bovine muscle.
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