轨道轨道
化学
色谱法
质谱法
萃取(化学)
三级四极质谱仪
洗脱
基质(化学分析)
分辨率(逻辑)
分析化学(期刊)
串联质谱法
选择性反应监测
计算机科学
人工智能
作者
Yan Xu,Haixia Li,Jian Liang,Jina Ma,Juan Yang,Xiaojuan Zhao,Wei Zhao,Weidong Bai,Xiaofang Zeng,Hao Dong
出处
期刊:Food Chemistry
[Elsevier]
日期:2021-05-18
卷期号:361: 130147-130147
被引量:68
标识
DOI:10.1016/j.foodchem.2021.130147
摘要
Triple quadrupole mass spectrometry has been the main technique for HAAs analysis in recent decade, while it requires extensive optimization of compound-dependent parameters. A novel method based on HPLC–Q-Orbitrap-HRMS was developed firstly for simultaneous determination of eighteen HAAs. Extraction and purification conditions were optimized and the developed method was validated in terms of linearity, accuracy and precision. Results indicated eighteen HAAs and two internal standards could be separated in 12 min using a gradient elution program. The full MS/dd-MS2 scan was adopted for analysis, which indicated favorable recoveries (71.3–114.8%) along with LODs and LOQs in the ranges of 0.02–0.6 and 0.05–2.0 μg/kg, respectively. Internal standards used for calibration could effectively reduce quantification errors produced by matrix effects. The validated method was successfully applied for HAAs analysis in roasted and pan-fried meat and was confirmed to be an alternative method when triple quadrupole mass spectrometry is absent in lab.
科研通智能强力驱动
Strongly Powered by AbleSci AI