Electrochemiluminescent determination of prostate-specific antigen using Au@(MoS2/GO/o-MWNTs) nanohybrids as co-reaction accelerator and hyperbranched hybridization chain reaction for signal amplification

Three-dimensional flowerlike Au@(MoS2/GO/o-MWNTs) nanohybrids (abbreviated as AMGMs) were synthesized and then introduced into an electrochemiluminescence (ECL) system as a new co-reaction accelerator for the ultrasensitive prostate-specific antigen (PSA). The AMGMs not only served as a substrate with good conductivity and a large specific surface area for loading abundant primary antibodies but also acted as an effective co-reaction accelerator; the co-reaction accelerator could interact with a co-reactant rather than the luminophore to boost the generation of free radical intermediates, thereby producing abundant excited states of luminophores to amplify the ECL signal response. Additionally, an anticipated signal amplification strategy based on the hybridization chain reaction (HCR) was developed by gathering a large amount of a DNA initiator on gold nanoparticles. These gathered DNA initiators could generate multiple DNA concatemers and attach more signal molecules, which resulted in outstanding exponential signal amplification. Consequently, the ECL immunosensor demonstrated high sensitivity, with a linear range from 0.1 pg mL−1 to 50 ng mL−1 and a detection limit of 0.028 pg mL−1. In addition, the immunosensor displayed excellent stability and selectivity. It was evaluated by analyzing human serum sample. The recovery obtained was 98.80–112.00% and the RSD was 1.73–3.12%, indicating the immunosensor could be applied to the simultaneous detection of PSA in human serum samples.