高铁血红蛋白
过氧化氢
氧化应激
氧化磷酸化
活性氧
化学
氧气
纳米颗粒
表面改性
儿茶酚
毒性
生物物理学
血红蛋白
核化学
生物化学
材料科学
纳米技术
有机化学
生物
物理化学
作者
Quan Wang,Ruirui Zhang,Guoxing You,Jilin Hu,Penglong Li,Ying Wang,Jun Zhang,Yan Wu,Lian Zhao,Hong Zhou
标识
DOI:10.1080/21691401.2018.1459636
摘要
Oxidative toxicity has impeded the development of haemoglobin-based oxygen carriers (HBOCs) by causing methaemoglobin (MetHb) formation and inducing oxidative stress. In our previous work, polydopamine-coated haemoglobin (Hb-PDA) nanoparticles have been designed and synthesized with the capacity to reduce oxidative toxicity. In this investigation, the mass ratio of dopamine (DA) to haemoglobin (Hb) and the pH value are found to be the primary factors that influence preparation of Hb-PDA nanoparticles. X-ray photoelectron spectroscopy showed that the catechol groups of DA play a crucial role in the modification of Hb surface. Hb-PDA nanoparticles were found to exhibit oxidative protection from hydrogen peroxide (H2O2) and the change of mitochondrial membrane potential showed that the Hb-PDA nanoparticles reduced H2O2-induced apoptosis. It is demonstrated that modification of PDA could maintain the oxygen-release capacity of Hb. These findings confirm that Hb-PDA nanoparticles possess restrained oxidative toxicity and preserve oxygen-release capacity.
科研通智能强力驱动
Strongly Powered by AbleSci AI