Fabrication of Glutaraldehyde Vapor Treated PVA/SA/GO/ZnO Electrospun Nanofibers with High Liquid Absorbability for Antimicrobial of Staphylococcus aureus

In this study, we aim to develop organic-inorganic hybrid nanofibers containing high moisture retention and good mechanical performance as an antimicrobial dressing platform. The main theme of this work focuses on several technical tasks including (a) the electrospinning process (ESP) to produce organic polyvinyl alcohol/sodium alginate (PVA/SA) nanofibers with an excellent diameter uniformity and fibrous orientation, (b) the fabrication of inorganic nanoparticles (NPs) as graphene oxide (GO) and ZnO NPs to be added to PVA/SA nanofibers for enhancement of the mechanical properties and an antibacterial function to Staphylococcus aureus (S. aureus), and then (c) the crosslinking process for PVA/SA/GO/ZnO hybrid nanofibers in glutaraldehyde (GA) vapor atmosphere to improve the hydrophilicity and moisture absorption of specimens. Our results clearly indicate that the uniformity nanofiber with 7 wt% PVA and 2 wt% SA condition demonstrates 199 ± 22 nm in diameter using an electrospinning precursor solution of 355 cP in viscosity by the ESP process. Moreover, the mechanical strength of nanofibers was enhanced by 17% after the handling of a 0.5 wt% GO nanoparticles addition. Significantly, the morphology and size of ZnO NPs can be affected by NaOH concentration, where 1 M NaOH was used in the synthesis of 23 nm ZnO NPs corresponding to effective inhibition of S. aureus strains. The PVA/SA/GO/ZnO mixture successfully performed an antibacterial ability with an 8 mm inhibition zone in S. aureus strains. Furthermore, the GA vapor as a crosslinking agent acting on PVA/SA/GO/ZnO nanofiber provided both swelling behavior and structural stability performance. The swelling ratio increased up to 1.406%, and the mechanical strength was 1.87 MPa after 48 h of GA vapor treatment. Finally, we successfully synthesized the hybrid nanofibers of GA-treated PVA/SA/GO/ZnO accompanied with high moisturizing, biocompatibility, and great mechanical properties, which will be a novel multi-functional candidate for wound dressing composites for patients receiving surgical operations and first aid treatments.