Lactobacillus juensis sp. nov. and Lactobacillus rizhaonensis sp. nov., isolated from the gut of honeybee (Apis mellifera)

Four lactic acid bacteria, designated F690 T , F697, F790 T and F769-2, were isolated from the gut of honeybee ( Apis mellifera ). Results of 16S rRNA gene sequence analysis indicated that strains F690 T and F697 were phylogenetically related to the type strains of Lactobacillus kimbladii , Lactobacillus laiwuensis , Lactobacillus kullabergensis and Lactobacillus huangpiensis , having 99.1–99.6 % 16S rRNA gene sequence similarities; and that strains F790 T and F769-2 were most closely related to the type strain of Lactobacillus melliventris , having 99.2–99.3 % 16S rRNA gene sequence similarities. The phylogenies based on concatenated pheS , rpoA , gyrB , hsp60 , recA , rpoB and tuf sequences and based on whole genome sequences were identical to that based on 16S rRNA gene sequences. Strains F690 T and F697 exhibited the highest average nucleotide identity (ANI; 92.1–93.2 %), digital DNA–DNA hybridization (dDDH; 50–50.1 %) and average amino acid identity (AAI; 94.9–95.1 %) values with L. kimbladii Hma2N T . Strains F790 T and F769-2 had the highest ANI (93.1–94 %), dDDH (54.4 %) and AAI (94.4–94.7 %) values with L. melliventris Hma8N T . Based upon the data obtained in the present study, two novel species, Lactobacillus juensis sp. nov. and Lactobacillus rizhaonensis sp. nov., are proposed and the type strains are F690 T (=JCM 36259 T =CCTCC AB 2023131 T ) and F790 T (=JCM 36260 T =CCTCC AB 2023132 T ), respectively.