Molecular characterization of Alternaria burnsii causing blight of cumin using random amplified polymorphic DNA (RAPD) markers

RAPD 枯萎病 生物 DNA 链格孢 遗传学 植物 遗传多样性 医学 人口 环境卫生
作者
Sunaina Varma,Data Ram Kumhar,S. L. Godara
出处
期刊:Annals of phytomedicine [Marwah Infotech]
卷期号:12 (2) 被引量:1
标识
DOI:10.54085/ap.2023.12.2.117
摘要

Ten isolates of the cumin blight pathogen (Alternaria burnsii) were obtained from the major cumingrowing regions of Rajasthan state in India during the Rabi seasons of 2019-20 and 2020-21.The isolates were molecularly characterized using genomic DNA and random amplified polymorphic DNA (RAPD) analysis with five decamer primers from the OPA and OPE series.A total of ten isolates were chosen from the major cumin-growing areas of Rajasthan, and out of the ten RAPD primers used initially, only five were chosen based on their repeatability.The RAPD analysis showed 100% polymorphism, indicating genetic diversity among the isolates.Dendrogram analysis was performed to group the isolates based on their genetic relatedness, resulting in two major clusters designated C1 and C2.Cluster C1 contained nine subclusters, including Ab-RSR, Ab-OSN, Ab-BLR, Ab-BHML, Ab-RNIW, Ab-KKR, Ab-KSG, Ab-NKH, and Ab-BCWL, while cluster C2 had only one isolate, Ab-BLTR.The similarity matrix showed that Ab-BCWL had a higher similarity coefficient value of 1.000, indicating greater genetic similarity among the isolates in this subcluster, while Ab-BLTR had a lower similarity coefficient value of 0.222, indicating less genetic similarity with the other isolates.The similarity coefficient represents the degree of genetic similarity between the isolates, and its variation among the isolates provides insight into the genetic diversity of the cumin blight pathogen population in Rajasthan.The genetic diversity analysis provides crucial insights into the pathogen's population structure.This understanding of genetic clusters and sub-clusters will be essential for recognizing different virulent strains or variations that could influence disease severity and resistance patterns, ultimately leading to the development of more effective management strategies for cumin blight.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
糊涂的猎豹完成签到,获得积分10
1秒前
魏为维发布了新的文献求助10
1秒前
dkyt完成签到,获得积分10
3秒前
嵇如雪完成签到,获得积分10
3秒前
4秒前
量子星尘发布了新的文献求助10
4秒前
5秒前
zhouyunan完成签到,获得积分10
5秒前
竹子完成签到,获得积分10
6秒前
7秒前
练习时长两年半应助Yve采纳,获得10
7秒前
金枪鱼完成签到,获得积分10
7秒前
Hello应助谨言采纳,获得10
8秒前
8秒前
英俊的铭应助研友_LkD29n采纳,获得10
8秒前
徐佳达完成签到,获得积分10
9秒前
shenxian82133发布了新的文献求助10
12秒前
李傲寒发布了新的文献求助10
13秒前
Man发布了新的文献求助10
13秒前
hyz完成签到,获得积分10
15秒前
16秒前
科研通AI5应助予安采纳,获得10
17秒前
科研x完成签到,获得积分10
17秒前
19秒前
听说外面下雨了完成签到,获得积分10
20秒前
CipherSage应助李傲寒采纳,获得10
21秒前
刘YF发布了新的文献求助10
25秒前
25秒前
orixero应助ty采纳,获得10
27秒前
28秒前
拉长的冷霜完成签到,获得积分10
28秒前
30秒前
英姑应助ccmaxp采纳,获得10
30秒前
wu先生完成签到,获得积分10
33秒前
jmn发布了新的文献求助10
33秒前
33秒前
37秒前
37秒前
38秒前
大米完成签到,获得积分10
38秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3975543
求助须知:如何正确求助?哪些是违规求助? 3519971
关于积分的说明 11200248
捐赠科研通 3256311
什么是DOI,文献DOI怎么找? 1798213
邀请新用户注册赠送积分活动 877446
科研通“疑难数据库(出版商)”最低求助积分说明 806338