Control of anthracnose (Colletotrichum gloeosporioides) growth in “Hass” avocado fruit using sachets filled with oregano oil-starch-capsules

Active antifungal packaging is a technological solution for reducing the postharvest losses of fruits and vegetables associated with phytopathogens. Anthracnose (Colletotrichum gloeosporioides) is the principal fungus that causes post-harvest avocado fruit decay. In this study, antifungal sachets filled with oregano oil-starch capsules were prepared, and their active effects were demonstrated on Hass avocado fruits. Oregano oil (31% of carvacrol) was encapsulated with corn starch by spray drying. Tyvek sachets (4 × 4 cm) filled with 80 (T1) and 160 mg (T2) of oregano oil-starch capsules (99.35 ± 1.86 mg/g−) were fabricated. The antifungal effects of the sachets were tested in vitro and in vivo using a humidity chamber (90–95% relative humidity (RH)) on fruits inoculated with anthracnose. The results showed that T1 and T2 inhibited 75.21 ± 2.81 and 100% in vitro growth of anthracnose at 25°C for 12 days. Furthermore, Hass avocado fruits stored in a humidity chamber at 25°C for 6 days showed that only T2 significantly (p<0.05) reduced the area of lesion produced by artificial inoculation of Hass avocado fruits with anthracnose. On average, the lesion area in the Hass avocado fruits treated with T2 was 13.94% smaller than that in the control fruit.