微分脉冲伏安法
适体
检出限
石墨烯
纳米棒
化学
线性范围
电化学
材料科学
纳米技术
组合化学
色谱法
循环伏安法
电极
分子生物学
物理化学
生物
作者
Yidan Zhang,Baoshan He,Renyong Zhao,Chunqi Bai,Yurong Zhang,Huali Jin,Min Wei,Wenjie Ren,Zhiguang Suo,Yiwei Xu
标识
DOI:10.1016/j.scitotenv.2022.157769
摘要
Herein, an aptasensor based on target-induced strand displacement (TISD) strategy was developed for sensitive detection of T-2 toxin. Gold [email protected] aminated manganese dioxide ([email protected]2-MnO2) exhibited excellent electrical conductivity and provided more binding sites for aptamer (Apt). Besides, polyethyleneimine-reduced graphene oxide/gold‑platinum core-shell nanorods composites (PEI-rGO/[email protected] NRs) were used to be carriers for signaling tags, as their sufficiently large specific surface area improved the loading capacity for signal molecules. In the presence of T-2, the Apt sequence was more inclined to form an Apt-T-2 complex, and the cDNA was displaced from the Apt-cDNA duplex, while the signal tag was released, resulting in a weakened MB signal, differential pulse voltammetry (DPV) was used to record the signal change. Under optimal conditions, the signal response of the constructed electrochemical aptasensor exhibited a good linear relationship with the concentration of T-2. The detection limit was 8.74 × 10−7 ng mL−1over a wide range of concentration from 5 × 10−6 ng mL−1 to 5 ng mL−1. Furthermore, the proposed aptasensor had excellent specificity, good stability and can be well applied to the detection of real samples. It provided a new avenue for the research and development of sensitive aptasensors in food detection and analysis.
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