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Carpesii fructus extract exhibits neuroprotective effects in cellular and Caenorhabditis elegans models of Parkinson's disease

神经保护 秀丽隐杆线虫 帕金森病 疾病 神经科学 生物 传统医学 医学 遗传学 内科学 基因
作者
Feng‐Dan Zhu,Sheng Wang,Dalian Qin,Xiaohui Su,Lu Yu,Jianming Wu,Betty Yuen Kwan Law,Min‐Song Guo,Chong‐Lin Yu,Xiaogang Zhou,Anguo Wu
出处
期刊:CNS Neuroscience & Therapeutics [Wiley]
被引量:2
标识
DOI:10.1111/cns.14515
摘要

Abstract Objective Parkinson's disease (PD) is a debilitating neurodegenerative disorder characterized by the progressive loss of dopaminergic neurons in the substantia nigra. Despite extensive research, no definitive cure or effective disease‐modifying treatment for PD exists to date. Therefore, the identification of novel therapeutic agents with neuroprotective properties is of utmost importance. Here, we aimed to investigate the potential neuroprotective effects of Carpesii fructus extract (CFE) in both cellular and Caenorhabditis elegans ( C. elegans ) models of PD. Methods The neuroprotective effect of CFE in H 2 O 2 ‐ or 6‐OHDA‐induced PC‐12 cells and α‐synuclein‐overexpressing PC‐12 cells were investigated by determining the cell viability, mitochondrial damage, reactive oxygen species (ROS) production, apoptosis, and α‐synuclein expression. In NL5901, BZ555, and N2 worms, the expression of α‐synuclein, motive ability, the viability of dopaminergic neurons, lifespan, and aging‐related phenotypes were investigated. The signaling pathway was detected by Western blotting and validated by employing small inhibitors and RNAi bacteria. Results In cellular models of PD, CFE significantly attenuated H 2 O 2 ‐ or 6‐OHDA‐induced toxicity, as evidenced by increased cell viability and reduced apoptosis rate. In addition, CFE treatment suppressed ROS generation and restored mitochondrial membrane potential, highlighting its potential as a mitochondrial protective agent. Furthermore, CFE reduced the expression of α‐synuclein in wide type (WT)‐, A53T‐, A30P‐, or E46K‐α‐synuclein‐overexpressing PC‐12 cells. Our further findings reveal that CFE administration reduced α‐synuclein expression and improved its induced locomotor deficits in NL5901 worms, protected dopaminergic neurons against 6‐OHDA‐induced degeneration in BZ555 worms, extended lifespan, delayed aging‐related phenotypes, and enhanced the ability of stress resistance in N2 worms. Mechanistic studies suggest that the neuroprotective effects of CFE may involve the modulation of the MAPK signaling pathway, including ERK, JNK, and p38, whereas the interference of these pathways attenuated the neuroprotective effect of CFE in vitro and in vivo. Conclusion Overall, our study highlights the potential therapeutic value of CFE as a neuroprotective agent in the context of PD. Furthermore, elucidation of the active compounds of CFE will provide valuable insights for the development of novel therapeutic strategies for PD.
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