亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Metabolite Cross-Feeding Promoting NADH Production and Electron Transfer during Efficient SMX Biodegradation by a Denitrifier and S. oneidensis MR-1 in the Presence of Nitrate

舍瓦内拉 化学 脱氮副球菌 生物化学 辅因子 代谢物 硝酸盐 亚硝酸盐 血红素 电子转移 电子传输链 生物降解 细胞色素 环境化学 细菌 有机化学 生物 遗传学
作者
Chunxia Zhao,Xu Duan,Chao Liu,Haining Huang,Meirou Wu,Xuemeng Zhang,Yinguang Chen
出处
期刊:Environmental Science & Technology [American Chemical Society]
卷期号:57 (46): 18306-18316 被引量:12
标识
DOI:10.1021/acs.est.2c09341
摘要

Antibiotics often coexist with other pollutants (e.g., nitrate) in an aquatic environment, and their simultaneous biological removal has attracted widespread interest. We have found that sulfamethoxazole (SMX) and nitrate can be efficiently removed by the coculture of a model denitrifier (Paracoccus denitrificans, Pd) and Shewanella oneidensis MR-1 (So), and SMX degradation is affected by NADH production and electron transfer. In this paper, the mechanism of a coculture promoting NADH production and electron transfer was investigated by proteomic analysis and intermediate experiments. The results showed that glutamine and lactate produced by Pd were captured by So to synthesize thiamine and heme, and the released thiamine was taken up by Pd as a cofactor of pyruvate and ketoglutarate dehydrogenase, which were related to NADH generation. Additionally, Pd acquired heme, which facilitated electron transfer as heme, was the important composition of complex III and cytochrome c and the iron source of iron sulfur clusters, the key component of complex I in the electron transfer chain. Further investigation revealed that lactate and glutamine generated by Pd prompted So chemotactic moving toward Pd, which helped the two bacteria effectively obtain their required substances. Obviously, metabolite cross-feeding promoted NADH production and electron transfer, resulting in efficient SMX biodegradation by Pd and So in the presence of nitrate. Its feasibility was finally verified by the coculture of an activated sludge denitrifier and So.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
冰红茶完成签到 ,获得积分10
1秒前
123完成签到 ,获得积分10
5秒前
调研昵称发布了新的文献求助10
22秒前
小冯完成签到 ,获得积分10
29秒前
30秒前
41秒前
linllll完成签到,获得积分10
42秒前
今后应助zhhha采纳,获得10
42秒前
无聊又夏完成签到,获得积分10
46秒前
pin给pin的求助进行了留言
55秒前
release枫完成签到,获得积分10
56秒前
1分钟前
wish完成签到 ,获得积分10
1分钟前
1分钟前
欢欢发布了新的文献求助10
1分钟前
文欣完成签到 ,获得积分10
1分钟前
zhhha发布了新的文献求助10
1分钟前
暴躁的鱼完成签到 ,获得积分10
1分钟前
狐狸萌萌哒完成签到 ,获得积分10
1分钟前
欢欢完成签到,获得积分10
1分钟前
1分钟前
1分钟前
spark810应助科研通管家采纳,获得10
1分钟前
顾矜应助科研通管家采纳,获得10
1分钟前
spark810应助科研通管家采纳,获得10
1分钟前
科研通AI2S应助科研通管家采纳,获得30
1分钟前
吴炜华发布了新的文献求助10
1分钟前
晴朗发布了新的文献求助10
1分钟前
可爱的函函应助sping采纳,获得10
1分钟前
2分钟前
空岛与影发布了新的文献求助30
2分钟前
吴炜华发布了新的文献求助10
2分钟前
2分钟前
充电宝应助22222采纳,获得30
2分钟前
空岛与影完成签到,获得积分10
2分钟前
2分钟前
2分钟前
22222发布了新的文献求助30
2分钟前
菲莳完成签到 ,获得积分10
2分钟前
热舞特完成签到,获得积分10
2分钟前
高分求助中
Sustainability in Tides Chemistry 1500
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
Threaded Harmony: A Sustainable Approach to Fashion 799
Livre et militantisme : La Cité éditeur 1958-1967 500
Retention of title in secured transactions law from a creditor's perspective: A comparative analysis of selected (non-)functional approaches 500
"Sixth plenary session of the Eighth Central Committee of the Communist Party of China" 400
New China Forges Ahead: Important Documents of the Third Session of the First National Committee of the Chinese People's Political Consultative Conference 400
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3056425
求助须知:如何正确求助?哪些是违规求助? 2713046
关于积分的说明 7434315
捐赠科研通 2357999
什么是DOI,文献DOI怎么找? 1249197
科研通“疑难数据库(出版商)”最低求助积分说明 606972
版权声明 596195